Kristopher: We did and still does next things: 1. Fixation in 10% NBF not less than 24 hours at RT or at 37-40oC overnight (from 16 PM to 8 AM). 2. Rinse with tap water 15-20 mins at RT. 3. Keep in 70% isopropanol at RT before processing as need long without any adverse effects.
We totally excluded ethanol as dhydratant. If you need our processing schedule, please let me know. All the best, Maxim Peshkov, Russia, Taganrog. mailto:maxim...@mail.ru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
