John,
OUCH! > From: jkier...@uwo.ca > To: syi...@ucalgary.ca > Date: Sat, 28 Nov 2009 01:33:55 -0500 > Subject: Re: [Histonet] a basic question about immunohistochemistry > CC: histonet@lists.utsouthwestern.edu > > Dear Dr Inan, > > Anyone can fix, process and do immunohistochemistry on parts of old, dead > "lab" rats. What you see will have to be compared with comparably > immunostained sections of old, dead "normal" rat tissues. This is bad > science! > > You need to repeat the experiment. Do the work yourself and don't rely on > untrained or uneducated "staff". > > John Kiernan > Anatomy, UWO > London, Canada > = = = > ----- Original Message ----- > From: Salim Yalcin Inan <syi...@ucalgary.ca> > Date: Friday, November 27, 2009 17:46 > Subject: [Histonet] a basic question about immunohistochemistry > To: histonet@lists.utsouthwestern.edu > > > Dear All, > > > > Because I am new in immunohistochemistry, I have a basic > > question about it. > > What if your rat dies in the evening or in the weekend, which > > you are doing > > a chronic experiment and need to collect brain tissue for > > immunohistochemistry? And let's say, the staff did not noticed > > it to inform > > you on time. Several hours passed since your rat died. There is > > no way to do > > perfusion. Is it still possible to do immunohistochemistry? > > Thank you very much in advance. > > > > Best regards, > > > > Salim Yalcin Inan, Ph.D. > > (post-doctoral fellow) > > Department of Clinical Neurosciences > > University of Calgary > > syi...@ucalgary.ca > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
