I am afraid that any IHC that you will attempt to do will not serve for your original experimental purposes of having a perfused brain. I am afraid you will have to start all over again. At least now you know a probable survival time, so start the new experiment in a way that the rat is "supposed to die" from a Tuesday on (during the work schedule). René J.
--- On Fri, 11/27/09, Salim Yalcin Inan <syi...@ucalgary.ca> wrote: From: Salim Yalcin Inan <syi...@ucalgary.ca> Subject: [Histonet] a basic question about immunohistochemistry To: histonet@lists.utsouthwestern.edu Date: Friday, November 27, 2009, 5:44 PM Dear All, Because I am new in immunohistochemistry, I have a basic question about it. What if your rat dies in the evening or in the weekend, which you are doing a chronic experiment and need to collect brain tissue for immunohistochemistry? And let's say, the staff did not noticed it to inform you on time. Several hours passed since your rat died. There is no way to do perfusion. Is it still possible to do immunohistochemistry? Thank you very much in advance. Best regards, Salim Yalcin Inan, Ph.D. (post-doctoral fellow) Department of Clinical Neurosciences University of Calgary syi...@ucalgary.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
