Since Immuno-cal is a commercial decalcifyer it probably was developed after testing and is probably good, but I always used EDTA as a chelating agent for IHC. René J.
--- On Mon, 12/14/09, Michele Wich <mw...@7thwavelabs.com> wrote: From: Michele Wich <mw...@7thwavelabs.com> Subject: [Histonet] IHC macrophage marker for demineralized bone To: histonet@lists.utsouthwestern.edu Date: Monday, December 14, 2009, 4:30 PM Can anyone recommend an antibody against rat macrophages which will work in FFPE de-mineralized bone sections? F4/80 possibly? Is Immuno-cal the best decalcifying solution for IHC procedures? I would greatly appreciate any suggestions. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
