You will probably have to use EDTA, there is a whole subset of rat macrophages, ED1, ED2, ED3 (Serotec has these) and you should know which macrophage subset you need to see. Sorry, F4/80 is for mouse macrophages not rat macrophages.
Rat macrophages may be very sensitive to any type of acid decalcification although people report successful staining with formalin fixed paraffin embedded tissues which means you could use EDTA instead of an acid. If you go to SEROTEC, and look up the antibody data sheets, you will see what application is going to work best for rat macrophages e.g. FFPE, frozen sections only, or some other fixation such as PLP (paraformaldehyde/lysine/periodate). Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Monday, December 14, 2009 2:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC macrophage marker for demineralized bone Can anyone recommend an antibody against rat macrophages which will work in FFPE de-mineralized bone sections? F4/80 possibly? Is Immuno-cal the best decalcifying solution for IHC procedures? I would greatly appreciate any suggestions. __________ Information from ESET Smart Security, version of virus signature database 4684 (20091213) __________ The message was checked by ESET Smart Security. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet