Hi - someone emailed me about my methodology, but I cant find the email, so here it is: I tried it @ 1:10, 1:25 and 1:50. All seem viable dilutions for the FFPE xenografts. I used BIocare DIVA (EDTA) HIER in decloaking chamber, primary ab for 2 hours @ RT, Biocare Rat-on-Mouse HRP polymer kit (15 minutes each) + DAB. The path labs (for my company) that are in California and Seattle used the same retreival but different detection methods and got similar results.
Kim From:ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Kim Merriam Sent: Wednesday, January 27, 2010 9:23 AM To: Histonet; ih...@googlegroups.com Subject: [IHCRG] Dianova rat anti-mouse CD31 Hi All, I know there was some buzz a while back about this antibody. I have tested it on mouse FFPE xenografts, and I must say, it looks very promising. I compared 2 pieces of the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was fixed in NBF. The NBF-fixed tumor actually looks better! A couple of other folks that are working at the west coast sites of my company have had similar results. We are all very encouraged. I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
