Thank you Kim, that does sound very promising, I still have not gotten around to trying my sample of this, your work will be very helpful when I do.
Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pru...@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _____ From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of James Watson Sent: Thursday, January 28, 2010 8:19 AM To: 'kmerriam2...@yahoo.com'; Histonet; ih...@googlegroups.com Subject: RE: [IHCRG] Dianova rat anti-mouse CD31 I just got some of this antibody in an will be setting it up on the Ventana Discovery Thank you for the information on your protocol. I will let you know how it goes. Jamie James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 <mailto:858-812-1915jwat...@gnf.org> jwat...@gnf.org From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Kim Merriam Sent: Thursday, January 28, 2010 6:46 AM To: Histonet; ih...@googlegroups.com Subject: Fw: [IHCRG] Dianova rat anti-mouse CD31 Hi - someone emailed me about my methodology, but I cant find the email, so here it is: I tried it @ 1:10, 1:25 and 1:50. All seem viable dilutions for the FFPE xenografts. I used BIocare DIVA (EDTA) HIER in decloaking chamber, primary ab for 2 hours @ RT, Biocare Rat-on-Mouse HRP polymer kit (15 minutes each) + DAB. The path labs (for my company) that are in California and Seattle used the same retreival but different detection methods and got similar results. Kim From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Kim Merriam Sent: Wednesday, January 27, 2010 9:23 AM To: Histonet; ih...@googlegroups.com Subject: [IHCRG] Dianova rat anti-mouse CD31 Hi All, I know there was some buzz a while back about this antibody. I have tested it on mouse FFPE xenografts, and I must say, it looks very promising. I compared 2 pieces of the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was fixed in NBF. The NBF-fixed tumor actually looks better! A couple of other folks that are working at the west coast sites of my company have had similar results. We are all very encouraged. I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet