In response to the "interested in other freezing techniques" part of
Laurie's query: We snap freeze by throwing a cyrovial of tissue (or a
tissue piece wrapped in foil) into some liquid nitrogen for a couple
minutes (vials will float, foil-wrapped pieces will sink). For controlled
freezing of OCT-embedded tissues, I've frozen isopentane by pouring some in
a liquid-nitrogen proof shallow container (such as a pipet-tip box lid) and
floating it on the liquid nitrogen til frozen. I guess this works if you're
processing a relatively small number of samples at a time, which, if you
work in a hospital, sounds like you may not be. Additionally, we've made
alcohol-dry ice slurries in styrofoam containers in which to freeze
OCT-embedded tissues.
Regards,
Merced
--On Thursday, February 25, 2010 10:12 AM -0500 Michael W
illiams <mjwilli...@scilogex.com> wrote:
It can be purchased directly from 3M. You may also find some local
suppliers.
Michael Williams
mjwilli...@scilogex.com
SCILOGEX, LLC
TF: (877) SCILOGEX
T: (860) 828-5614
F: (860) 828-5389
www.SCILOGEX.com
-----Original Message-----
From: Houston, Ronald [mailto:ronald.hous...@nationwidechildrens.org]
Sent: Thursday, February 25, 2010 9:42 AM
To: Michael Williams; Laurie Colbert
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Snap Freezing Tissue
Who supplies the 3MT Novec Engineered Fluid HFE-7100?
Thanks
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
Williams
Sent: Thursday, February 25, 2010 8:55 AM
To: 'Laurie Colbert'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Snap Freezing Tissue
Hi Laurie,
We distribute Bright's Clini-RF. We hear good reports about using 3MT
NovecT
Engineered Fluid HFE-7100 which is inert and has an excellent
environmental,
health and safety profile.
Regards,
Michael Williams
mjwilli...@scilogex.com
SCILOGEX, LLC
TF: (877) SCILOGEX
T: (860) 828-5614
F: (860) 828-5389
www.SCILOGEX.com
-----Original Message-----
From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com]
Sent: Wednesday, February 24, 2010 4:55 PM
To: histonet@lists.utsouthwestern.edu
Cc: Steve Pike
Subject: [Histonet] Snap Freezing Tissue
I was wondering how others are snap freezing tissue? We have been using
a histobath (a refrigerator device that cools isopentane for freezing
tissue), but it has died. I would love to have another Histobath, but
they are no longer carried by the vendor that I originally bought mine
from. Does anyone know where I can get either a new or refurbished
Histobath (vendor calls are welcome)? I'm also interested in other
freezing techniques.
Laurie Colbert
Huntington Hospital
Pasadena, CA
(626) 397-8620
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Merced M Leiker
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Cardiovascular Medicine
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