I think there is confusion regarding the original question posed. They were not asking about the HPS stain. Some hospitals use an eosin/saffron stain in their routine H&E. I had another lab recently asking about the formula to prepare this solution instead of eosin. Any advise?
Diana -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Wednesday, March 17, 2010 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HPS Stain Hi Sheila, HPS (Hematoxylin-Phloxine-Saffron) is the routine stain we use. There are a few institutions in Canada that also still use this stain; around Ottawa and a few other places in Ontario and Quebec. Perhaps one of these institutions sent slides to your pathologist for referral/consult? It is quite a nice trichrome stain, but somewhat more complex and expensive, uses more staining dishes, and it's sometimes tricky to get a proper phloxine/saffron balance. Due to the use of H&E being more widespread, our pathology residents usually ask for H&E slides for their files when preparing for exams. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet