Hello Fellow Histologists,
I am writing because I need information on how to process calcified bone for cryomicrotomy. I am working on an animal bone study that will require cryosectioning of calcified bone to stain for fat (Oil Red O). Does anyone have any information on this topic that would like to share? A procedure, cryostat used, and mounting medium. Thanks in advance, Fanny Maria V. De Guzman| Histology Technician I Main 908.947.1100 Fax 908.947.1085 Direct 908.947.1482 Email mdeguz...@lifecell.com Mobile 732.688.1386 www.Lifecell.com LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, March 17, 2010 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 76, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. UNSUBSCRIBE (Vanessa Avalos) 2. Exciting Histotech Job in New England!! (Alisha Dynan) 3. Re: UNSUBSCRIBE (kim.dona...@bhcpns.org) 4. HT/HTL position Gainesville. FL (Konni Black) 5. Re: proficiency testing for Muscle EHC- cont'd. (Barone, Carol ) 6. Spencer AO 820 microtome questions (Scott Parker) 7. Job Openings in Phoenix, Arizona (Turner, Mark) 8. Lab Assistant Position in Phoenix, AZ (Turner, Mark) 9. Saffron/Eosin as a counterstain for H&E??? (sheila adey) 10. RE: Saffron/Eosin as a counterstain for H&E??? (Liz Chlipala) 11. NY License (Jeffrey Silverman) 12. (no subject) (Jeffrey Silverman) 13. CAP cancer protocols and checklists (Carol Bryant) 14. (no subject) (Jeffrey Silverman) 15. Re: Removing Yellow color from slides refixed in Bouin's solution (Joseph Saby) 16. RE: (no subject) (Sherwood, Margaret ) 17. RE: (no subject) (WILLIAM DESALVO) 18. Re: Spencer AO 820 microtome questions (Michael Folsom) 19. Re: Saffron/Eosin as a counterstain for H&E??? (John Kiernan) 20. RE: Superfrost Plus Slides (Cathy) 21. problem in IF stains (Aazath Raj) 22. Accessioning Similar Samples (Breeden, Sara) 23. Re: Accessioning Similar Samples (dkb...@chs.net) 24. RE: Accessioning Similar Samples (Weems, Joyce) 25. Mc Coy fixative (Cynthia Pyse) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Mar 2010 10:01:22 -0700 From: "Vanessa Avalos" <vava...@allergydermatology.com> Subject: [Histonet] UNSUBSCRIBE To: "'HISTONET LISTS'" <histonet@lists.utsouthwestern.edu> Message-ID: <001001cac52a$4ef4ba00$ecde2e...@com> Content-Type: text/plain; charset="UTF-8" I can only speak for myself but yes, I am aware of all steps to be taken in unsubscribing. Have followed them more than once but have been unsuccessful. So yes, my frustration as lead me to write IN ALL CAPS!!!!! :) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, March 16, 2010 7:53 AM To: Dimitri Scholz Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) both of you are not allowed to use the internet anymore. because you can't follow instructions. WE CAN'T UNSUBSCRIBE YOU. ONLY YOU CAN. A state-imposed metaphysic or religion should be opposed, if necessary at pistol-point. We must fight for variety if we fight at all. The uniform is as dull as a sculptured egg. --Lawrence Durrell, Consequential Data, "Balthazar" On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz <dimitri.sch...@ucd.ie> wrote: > Me too! > > Dimitri Scholz, PhD, Dr. Sci. > Director of Biological Imaging > Conway Institute > University College Dublin (UCD) > Belfield, Dublin 4 > Ireland > Tel: +353-1 716 6736 > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa > Gutierrez > Sent: 16 March 2010 14:21 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM > ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE > RULES GETS ME OFF! > > THANK YOU > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: 16 Mar 2010 13:40:23 -0400 From: Alisha Dynan <ali...@ka-recruiting.com> Subject: [Histonet] Exciting Histotech Job in New England!! To: histonet@lists.utsouthwestern.edu Message-ID: <1516952045.1268761223703.javamail.cfserv...@webserver54> Content-Type: text/plain; charset="utf-8" Hi Histotechs, I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great job opportunities in various areas across the country. One particular client that I am working with is a financially sound, medically advanced, full-service hospital in New England. My client is looking for both a HTL(ASCP) certified histotechnologist and a HT(ASCP) certified histotech for their hospital. Both positions would work a day shift. My client is offering a very competitive compensation package, full benefits, and relocation assistance. If interested, please email me a copy of your resume and follow-up with a call to learn more! Below is a list of some of the great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Current Opportunities: CT - Histology Operations Manager NY - New York City - Histotech 3rd shift NV -Las Vegas - Histotech 3rd shift NV Las Vegas - Histology Supervisor - 3rd shift PA - Cytology Supervisor CA - Palm Springs - Histotech - 1st shift CA - Southern - Histotech VA - Histotech 2nd shift NH - HTL and HT GA - Atlanta - Histotech GA - Atlanta - Pathology Coordinator (HT or CT) NV - Pathologist Assistant TN - Pathologist Assistant ND - Pathologist Assistant GA - Pathologist Assistant If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ------------------------------ Message: 3 Date: Tue, 16 Mar 2010 12:47:31 -0500 From: kim.dona...@bhcpns.org Subject: Re: [Histonet] UNSUBSCRIBE To: "Vanessa Avalos" <vava...@allergydermatology.com> Cc: 'HISTONET LISTS' <histonet@lists.utsouthwestern.edu>, histonet-boun...@lists.utsouthwestern.edu Message-ID: <of178264b0.5bd13068-on862576e8.006155fc-862576e8.0061b...@bhcpns.org> Content-Type: text/plain; charset="US-ASCII" I have a suggestion. If you are extremely frustrated with getting all the emails that come from here and you are having a hard time unsubscribing, perhaps you could put a block on histonet so you cant receive emails from them until you get unsubscribed? Just a thought. Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Vanessa Avalos" <vava...@allergydermatology.com> Sent by: histonet-boun...@lists.utsouthwestern.edu 03/16/2010 12:01 PM To "'HISTONET LISTS'" <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] UNSUBSCRIBE I can only speak for myself but yes, I am aware of all steps to be taken in unsubscribing. Have followed them more than once but have been unsuccessful. So yes, my frustration as lead me to write IN ALL CAPS!!!!! :) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, March 16, 2010 7:53 AM To: Dimitri Scholz Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) both of you are not allowed to use the internet anymore. because you can't follow instructions. WE CAN'T UNSUBSCRIBE YOU. ONLY YOU CAN. A state-imposed metaphysic or religion should be opposed, if necessary at pistol-point. We must fight for variety if we fight at all. The uniform is as dull as a sculptured egg. --Lawrence Durrell, Consequential Data, "Balthazar" On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz <dimitri.sch...@ucd.ie> wrote: > Me too! > > Dimitri Scholz, PhD, Dr. Sci. > Director of Biological Imaging > Conway Institute > University College Dublin (UCD) > Belfield, Dublin 4 > Ireland > Tel: +353-1 716 6736 > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa > Gutierrez > Sent: 16 March 2010 14:21 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM > ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE > RULES GETS ME OFF! > > THANK YOU > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. ------------------------------ Message: 4 Date: Tue, 16 Mar 2010 10:59:16 -0700 From: "Konni Black" <kbl...@digestivehlth.com> Subject: [Histonet] HT/HTL position Gainesville. FL To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <dab581f471b84b6788798f6a3570a...@digestivehlth.com> Content-Type: text/plain; charset="Windows-1252" Fulltime HT/HTL opening in GI lab in Gainesville, Florida. Must have 3-5 years experience. Great lab. Flexible work schedule. Contact Konni Black at kbl...@digestivehlth.com or 253-503-2560. ------------------------------ Message: 5 Date: Tue, 16 Mar 2010 14:10:50 -0400 From: "Barone, Carol " <cbar...@nemours.org> Subject: [Histonet] Re: proficiency testing for Muscle EHC- cont'd. To: <histonet@lists.utsouthwestern.edu> Message-ID: <37e4bac017f57141af64faa5aeb04ce8033a7...@wlmmsx01.nemours.org> Content-Type: text/plain; charset="us-ascii" Histonetter's I only received one reply on my question regarding "what everyone is doing for proficiency testing of enzyme HC staining for muscle"...espeically for CLIA.compliance, now that I will lose my indepent reviewer. My thanks to Jan Minchew for her reply. I am working from that for now, though my sitiation somewhat different. .....but, I am finding it particularly frustrating finding an answer to this question over the last three months or more, that I have searched everywhere from the histonet to the internet toevery friend I have doing EHC. Is it required under CLIA? My auditor says yes..so I am trying to comply, though I cannot fiind it specifically in any CLIA reg. Who is the CLIA expert out there...and why is this not included in HQIP? Can someone shed more light. Thx. CB ------------------------------ Message: 6 Date: Tue, 16 Mar 2010 14:20:02 -0400 From: Scott Parker <spar...@vt.edu> Subject: [Histonet] Spencer AO 820 microtome questions To: histonet@lists.utsouthwestern.edu Message-ID: <ed777d3c1003161120l73339e82t8c699af45feb7...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Dear all, I have access to a Spencer AO 820 microtome (a "black beauty" in Histo-parlance) that is does not have a disposable blade holder nor a chuck for holding paraffin cassettes. The microtome otherwise appears to be in good condition. I would like to get suggestions for where I might be able to purchase a chuck and disposable blade holder for this classic model. Alternatively, would it be a better idea for me to put this piece of equipment on display in a glass case and instead purchase a newer microtome. My work involves relatively low volume, basic sectioning of paraffin blocks for teaching and research. I don't need anything too fancy, just a microtome that is reliable and appropriate for student researchers to use. Thank you in advance for your responses. Scott Scott L. Parker, Ph.D. Assistant Professor Biology Coastal Carolina University P.O. Box 261954 Conway, SC 29528-6054 Office Phone: (843)-349-2491 ------------------------------ Message: 7 Date: Tue, 16 Mar 2010 11:52:43 -0700 From: "Turner, Mark" <mtur...@carisdx.com> Subject: [Histonet] Job Openings in Phoenix, Arizona To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <c3b8b71fd81e9f418f7f4725046d395401b48...@s-phx-exc01.pathologypartners.intranet> Content-Type: text/plain; charset="us-ascii" Hello Histonetters! Caris MPI, a division of Caris Life Sciences, is looking for 2 histotechs to assist with a dramatically increasing workflow. We are a very dynamic IHC lab which is growing exponentially. The positions are day shift, Monday through Friday, with occasional weekends. The successful candidates will have HT (ASCP) certification, 1-2 years IHC experience, preferably with the Ventana and Dako platforms, and will enjoy being part of a fast paced, quality oriented team. Qualification in Immunohistochemistry (QIHC) is a plus. Caris MPI is located in sunny Phoenix, AZ and will be moving to a brand new lab in May 2010. Equipment is state of the art, and there is a corporate commitment toward quality. Caris has had a presence in the Phoenix area for 3 years and is continuing to grow. The area is the 5th largest metropolitan in the country and has available anything you need to make life more interesting. If you would like to grow with us, please submit your resume in confidence to mtur...@carisdx.com. Mark Turner, Ph. D., HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mtur...@carisdx.com ------------------------------ Message: 8 Date: Tue, 16 Mar 2010 11:57:47 -0700 From: "Turner, Mark" <mtur...@carisdx.com> Subject: [Histonet] Lab Assistant Position in Phoenix, AZ To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <c3b8b71fd81e9f418f7f4725046d395401b48...@s-phx-exc01.pathologypartners.intranet> Content-Type: text/plain; charset="us-ascii" Caris MPI, a division of Caris Life Sciences, is looking to hire a Lab Assistant for 2nd shift. This will be a support position for our team of histology professionals, and is a great entry level position into the laboratory setting. Some college is preferred, with a focus on the sciences. Opportunities for advancement abound, as this position is open due to our previous lab assistant moving into a histotech role. Caris MPI is located in sunny Phoenix, AZ and will be moving to a brand new lab in May 2010. Equipment is state of the art, and there is a corporate commitment toward quality. Caris has had a presence in the Phoenix area for 3 years and is continuing to grow. The area is the 5th largest metropolitan in the country and has available anything you need to make life more interesting. If you would like to grow with us, please submit your resume in confidence to mtur...@carisdx.com. Mark Turner, Ph. D., HT(ASCP) QIHC Supervisor IHC Target Now MPI Caris Life Sciences 445 N. 5th Street Phoenix, AZ 85004 Cell: 602-309-5084 Direct 602-358-8913 Fax: 602-358-8919 mtur...@carisdx.com ------------------------------ Message: 9 Date: Tue, 16 Mar 2010 15:04:23 -0400 From: sheila adey <sheila_a...@hotmail.com> Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? To: <histonet@lists.utsouthwestern.edu> Message-ID: <bay129-w13e52f735989673164655893...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Hello, Our pathologist said that he say H&E slides with a counterstain that had some amount of saffron in it? Is anyone familiar with this? Sheila Adey HT MLT _________________________________________________________________ Stay in touch. http://go.microsoft.com/?linkid=9712959 ------------------------------ Message: 10 Date: Tue, 16 Mar 2010 13:30:38 -0600 From: "Liz Chlipala" <l...@premierlab.com> Subject: RE: [Histonet] Saffron/Eosin as a counterstain for H&E??? To: "sheila adey" <sheila_a...@hotmail.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <ee33be5c905a3046a7ff8f58a64c8e4b100...@server.premierlab.local> Content-Type: text/plain; charset="us-ascii" Its hematoxylin, ploxine and saffron. Believe it or not it was the stain that we used at my first job back in 1973. We also fixed in Zenkers too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, March 16, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? Hello, Our pathologist said that he say H&E slides with a counterstain that had some amount of saffron in it? Is anyone familiar with this? Sheila Adey HT MLT _________________________________________________________________ Stay in touch. http://go.microsoft.com/?linkid=9712959_________________________________ ______________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 16 Mar 2010 14:07:54 -0700 (PDT) From: Jeffrey Silverman <pathmas...@yahoo.com> Subject: [Histonet] NY License To: rmo...@ameripath.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <12350.35531...@web111103.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii New York State Education Department- google it and you'll see a link for licensed professionals. Jeff Silverman ------------------------------ Message: 12 Date: Tue, 16 Mar 2010 14:14:57 -0700 (PDT) From: Jeffrey Silverman <pathmas...@yahoo.com> Subject: [Histonet] (no subject) To: cscam...@uci.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <161299.62124...@web111104.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Don't know what you mean by rinses, but 5 to at most 10 minutes in gently running tap water should do the trick. If not, the lithium carbonate is what's prescribed. Jeff Silverman ------------------------------ Message: 13 Date: Tue, 16 Mar 2010 17:19:33 -0400 From: Carol Bryant <cb...@lexclin.com> Subject: [Histonet] CAP cancer protocols and checklists To: "Histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <50da0c6b72976b4ab3a0fca04cc73dbf0567ddc...@exchangesb> Content-Type: text/plain; charset="us-ascii" I am interested in how you are implementing the CAP cancer protocols in your laboratory? Are you using synoptic reporting, dictating from the checklists, etc? Any information you can provide would be greatly appreciated. Thank you in advance for your comments and input. Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ------------------------------ Message: 14 Date: Tue, 16 Mar 2010 14:23:45 -0700 (PDT) From: Jeffrey Silverman <pathmas...@yahoo.com> Subject: [Histonet] (no subject) To: allison_sc...@hchd.tmc.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <190750.38557...@web111106.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Allison- That's our policy- don't accession like biopsies back to back. But if it is unavoidable, you can mark the second like biopsy case with a dab of yellow margin ink on at least one piece in each block and dictate that into the gross.? ie One fragment is marked in yellow for positive patient identification. Jeff Silverman- busy PA ------------------------------ Message: 15 Date: Tue, 16 Mar 2010 14:25:01 -0700 (PDT) From: Joseph Saby <saby_josep...@yahoo.com> Subject: Re: [Histonet] Removing Yellow color from slides refixed in Bouin's solution To: Cynthia Pyse <cp...@x-celllab.com>, cscam...@uci.edu, HistoNet <histonet@lists.utsouthwestern.edu> Message-ID: <619715.30232...@web113816.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Running tap water for 10 minutes should do the trick. Joe Saby ________________________________ From: Cynthia Pyse <cp...@x-celllab.com> To: cscam...@uci.edu; HistoNet <histonet@lists.utsouthwestern.edu> Sent: Tue, March 16, 2010 10:52:20 AM Subject: RE: [Histonet] Removing Yellow color from slides refixed in Bouin's solution I just place the slides into 80% ETOH for 2 minutes. Cindy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cscam...@uci.edu Sent: Monday, March 15, 2010 7:42 PM To: HistoNet Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's solution Hi Histonet, After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it very difficult to remove the yellow coloring. Rinses with water are not doing the trick. Does anyone have some advice on how to bring the slides back to a clear color so that I may proceed with the Masson Trichrome procedure? Thanks! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 16 Mar 2010 17:34:15 -0400 From: "Sherwood, Margaret " <msherw...@partners.org> Subject: RE: [Histonet] (no subject) To: "Jeffrey Silverman" <pathmas...@yahoo.com>, <cscam...@uci.edu> Cc: histonet@lists.utsouthwestern.edu Message-ID: <073ae2bea1c2ba4a8837ab6c4b943d9703e24...@phsxmb30.partners.org> Content-Type: text/plain; charset="us-ascii" We also just wash in running tap water and the yellow stain disappears. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Silverman Sent: Tuesday, March 16, 2010 5:15 PM To: cscam...@uci.edu Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Don't know what you mean by rinses, but 5 to at most 10 minutes in gently running tap water should do the trick. If not, the lithium carbonate is what's prescribed. Jeff Silverman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 17 Date: Tue, 16 Mar 2010 18:33:50 -0600 From: WILLIAM DESALVO <wdesalvo....@hotmail.com> Subject: RE: [Histonet] (no subject) To: <pathmas...@yahoo.com>, <allison_sc...@hchd.tmc.edu> Cc: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <blu103-w23701c4208d2f9a987d43291...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" An additional method to use to create positive identification when accessioning and grossing like specimens together when you must or just to increase the indetification factor for all specimens: Use the Davidson marking system and have the grossing person rotate the colors used to mark margins or small tissue by case and/or specimen and of course dictate to specific color used. Once they are in the habit, no two cases or specimens will have the same ink color and you increase quality assurance. William DeSalvo, B.S., HTL(ASCP) > Date: Tue, 16 Mar 2010 14:23:45 -0700 > From: pathmas...@yahoo.com > To: allison_sc...@hchd.tmc.edu > CC: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Hi Allison- > That's our policy- don't accession like biopsies back to back. > But if it is unavoidable, you can mark the second like biopsy case with a dab > of yellow margin ink on at least one piece in each block and dictate that > into the gross. ie One fragment is marked in yellow for positive patient > identification. > > Jeff Silverman- busy PA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Hotmail: Trusted email with powerful SPAM protection. http://clk.atdmt.com/GBL/go/210850553/direct/01/ ------------------------------ Message: 18 Date: Tue, 16 Mar 2010 18:53:05 -0600 From: Michael Folsom <mwfol...@rgbio.com> Subject: Re: [Histonet] Spencer AO 820 microtome questions To: Scott Parker <spar...@vt.edu> Cc: histonet@lists.utsouthwestern.edu Message-ID: <1268787185.6216.14.ca...@chico.322tulane.org> Content-Type: text/plain; charset="UTF-8" Scott: Does the chuck have a clamp in it? If so soak a piece of soft wood that fits in the chuck in 100% ETOH (pulling a vacuum on it would help) and transition into paraffin. Once its soaked in paraffin pull it out and allow to solidify. Your tissue will need to be flat embedded in a simple boat. Use a razor blade to cut it out but leave a good bit of paraffin behind and around it. Once its cool use an artists pallet knife (small) to melt the surface of the paraffin on the wood and the back of the block and weld in place with the melted paraffin. Re: the knife - they make a knife holder that holds either hi or low profile disposable blades which fits in the microtome's knife holder. One thing about the old AO - they are almost indestructible but never back up the crank because it can strip the gears. Good luck - Mike mwfol...@rgbio.cm On Tue, 2010-03-16 at 14:20 -0400, Scott Parker wrote: > Dear all, > > I have access to a Spencer AO 820 microtome (a "black beauty" in > Histo-parlance) that is does not have a disposable blade holder nor a chuck > for holding paraffin cassettes. The microtome otherwise appears to be in > good condition. I would like to get suggestions for where I might be able to > purchase a chuck and disposable blade holder for this classic model. > Alternatively, would it be a better idea for me to put this piece of > equipment on display in a glass case and instead purchase a newer microtome. > My work involves relatively low volume, basic sectioning of paraffin blocks > for teaching and research. I don't need anything too fancy, just a microtome > that is reliable and appropriate for student researchers to use. > > Thank you in advance for your responses. > > Scott > > > Scott L. Parker, Ph.D. > > Assistant Professor Biology > > Coastal Carolina University > > P.O. Box 261954 > > Conway, SC 29528-6054 > > > > Office Phone: (843)-349-2491 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Tue, 16 Mar 2010 23:05:55 -0400 From: John Kiernan <jkier...@uwo.ca> Subject: Re: [Histonet] Saffron/Eosin as a counterstain for H&E??? To: sheila adey <sheila_a...@hotmail.com> Cc: histonet@lists.utsouthwestern.edu Message-ID: <fbb4e5315571.4ba00...@uwo.ca> Content-Type: text/plain; CHARSET=US-ASCII There is Garvey,W (1991): Modification of the Mayer hematoxylin stain. J. Histotechnol. 14, 163-165. The title is rather confusing. Her method included a modified composition of Mayer's haemalum (ethanol instead of chloral hydrate, and less alum), and instead of eosin Y the counterstain was a solution containing phloxin B (CI45410, Acid red 92) and saffron (which is CI 75100, Natural yellow 6). The author stated that saffron bought from a health food store was OK and much less expensive than that bought from a lab vendor. This counterstain was used by Rostoker et al (2001) Nephrology Dialysis Transplantation 16:513-517 in a study of intestinal pathology in glomerulonephritis. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: sheila adey <sheila_a...@hotmail.com> Date: Tuesday, March 16, 2010 15:05 Subject: [Histonet] Saffron/Eosin as a counterstain for H&E??? To: histonet@lists.utsouthwestern.edu > > Hello, > > Our pathologist said that he say H&E slides with a counterstain > that had some amount of saffron in it? > > Is anyone familiar with this? > > Sheila Adey HT MLT > > > > _________________________________________________________________ > Stay in touch. > http://go.microsoft.com/?linkid=9712959_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 16 Mar 2010 20:34:43 -0700 From: "Cathy" <cf...@007group.com> Subject: RE: [Histonet] Superfrost Plus Slides To: "'Fahy, Claire'" <claire.f...@agriculture.gov.ie>, "'Lynn Lee'" <lynnlee2...@live.com> Cc: histonet@lists.utsouthwestern.edu Message-ID: <fb51fd708c6f4cf3b8bd1da7bd8b0...@your8ba846406f> Content-Type: text/plain; charset="iso-8859-1" Hi Claire, We experienced problems with our IHC staining the fall of 2008 and it was traced back to a problem with particular lot numbers of the Superfrost slides. It was difficult to track down due to the inconsistency of the problem and the variation in the problem. We finally spoke with our Ventana technical specialist and she was able to tie our problem to problems that other facilities were experiencing. You could try contacting your Ventana technical resource person; they may be able to help. One thing we found out was that the Superfrost slides are manufactured at one site and distributed to vendors under their own name. Cathy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fahy, Claire Sent: Tuesday, March 16, 2010 4:05 AM To: Lynn Lee Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Superfrost Plus Slides Hi Lynn Yes, our instrument is decontaminated and cleaned as per manual. There was some old threads on Histonet where some people had got varying staining between runs so I was wondering if anybody had found a solution to this problem.Maybe it is simply the slides? -----Original Message----- From: Lynn Lee [mailto:lynnlee2...@live.com] Sent: 16 March 2010 10:43 To: Fahy, Claire Subject: RE: [Histonet] Superfrost Plus Slides You could have contamination in the instrument. Has it been decontaminated quarterly per the instruction manual? I have never heard of uneven staining from plus slides. > Date: Tue, 16 Mar 2010 10:16:38 +0000 > From: claire.f...@agriculture.gov.ie > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Superfrost Plus Slides > > Hi > > I know this is in reply to an old threat but we are experiencing varying and uneven staining of our sections on the Ventana Benchmark.It is not confined to a perticular anitbody and doesn't happen all the time either. > > We have been told that it may be the slides.We currently use Thermo Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the Ventana IHC slides? > > Has anyone has experienced similar problems?If so,what have you done to resolve them. > > Thanks > > Claire fahy > Lab analyst, > Histopatholgy Lab > > Department of Agriculture, Fisheries and Food > > The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). > > An Roinn Talmha?ochta, Iascaigh agus Bia > > T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh ?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air, n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar ? do r?omhaire le do thoil. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. Learn More.<http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAG L:ON:WL:en-US:WM_HMP:032010_1> ________________________________ Department of Agriculture, Fisheries and Food The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). An Roinn Talmha?ochta, Iascaigh agus Bia T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh ?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air, n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar ? do r?omhaire le do thoil. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 17 Mar 2010 10:20:39 +0530 From: Aazath Raj <aaz...@hotmail.com> Subject: [Histonet] problem in IF stains To: <histonet@lists.utsouthwestern.edu> Message-ID: <snt137-w3417b9b444684f7a1b0fcac8...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Dear Friends, I am having a problem in IF stains on Renal Bx.In direct immunofloresence on the frozen sections does not have disered contrast and many of the cases its falsely negative. Can you people tell me the possible mistake which may cause these kind of effects on the IF stain. I dont see any gross mistakes on the Frozen sections after sectioning.All the temperature and other things are fine on the cryostate, but my pathologist feels that there may be any problem in section.will that have any effects on the IF stains.. Aazathraj. Technical Officer. Apollo Hospitals India. _________________________________________________________________ The latest songs, trailers and more http://video.in.msn.com/ ------------------------------ Message: 22 Date: Wed, 17 Mar 2010 06:04:10 -0600 From: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Subject: [Histonet] Accessioning Similar Samples To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <4d14f0fc9316dd41972d5f03c070908b02e46...@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Regarding the need to avoid accessioning similar samples consecutively, I addressed this last week with my bosses. Specifically, I asked that spleen specimens not be accessioned consecutively (because of shattering and static and the possibility of fragments being carried over); my response was that I should just not CUT the blocks consecutively. Not the point I was trying to make but typical in some cases when addressing the issues histotechs face, true? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 23 Date: Wed, 17 Mar 2010 08:24:55 -0400 From: dkb...@chs.net Subject: Re: [Histonet] Accessioning Similar Samples To: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Cc: histonet <histonet@lists.utsouthwestern.edu>, histonet-boun...@lists.utsouthwestern.edu Message-ID: <ofe1713805.e2a69779-on852576e9.00441eb7-852576e9.00443...@chs.net> Content-Type: text/plain; charset="US-ASCII" Sara, Unfortunately the response you got is more common than I would like to see. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 24 Date: Wed, 17 Mar 2010 10:21:34 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: RE: [Histonet] Accessioning Similar Samples To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <27648a6c5bc9b145813df5182f83ab45075...@itsssxm01v1.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We have agar based markers - red, blue, green, yellow, orange, black, that we include in the block for similar tissues - as we have several that be separated. It is noted in the gross and confirmed at micro that the markers are present. After we've gone through the colors once, we put two, three, etc. It has worked well for us. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 25 Date: Wed, 17 Mar 2010 11:00:35 -0400 From: "Cynthia Pyse" <cp...@x-celllab.com> Subject: [Histonet] Mc Coy fixative To: <histonet@lists.utsouthwestern.edu> Message-ID: <000001cac5e2$99201da0$cb6058...@com> Content-Type: text/plain; charset="us-ascii" Hi Histonetters I am having a senior moment. Can anyone tell me what McCoy fixative is made of and what it is used for? Thanks in advance for the help. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 76, Issue 24 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet