Hi Naira, We routinely do a 2 hour incubation in primary, but we make sure that more antibody is on the slide than would be necessary for most other reagents (500-600 ul). We have not had any issues of drying. If you are concerned about it, just make it 2 antibody steps instead of 1 on the machine.
Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ________________________________ From: "Margaryan, Naira" <nmargar...@childrensmemorial.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Thu, March 18, 2010 3:38:33 PM Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer Hi Histonetters! I have a stupid question but I Have to ask. Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? I know that slides should not be dry in any step of IHC. Thank you very much! Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet