Dear Histonetters, Thanks everyone for several good advices.
I'll try all suggestions: increase volume from 600ul to 800ul, add more primary antibody step and put hot water dishes inside of machine to keep humidity as high as possible. Hopefully it will work........... Many thanks, Naira -----Original Message----- From: Liz Chlipala [mailto:l...@premierlab.com] Sent: Thursday, March 18, 2010 3:05 PM To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Incubation of Ab more then 1 hour in autostainer We have done up to an hour but not longer, if I were you I would have two antibody steps and just to add some more reagent after an hour or 1.5 hours Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, March 18, 2010 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer Importance: High Hi Histonetters! I have a stupid question but I Have to ask. Does anyone perform an Incubation of Ab that required more then 1 hour (2-3 hours) in autostainer? Does autostainer keep slides wet or slides sometimes are getting dry? I know that slides should not be dry in any step of IHC. Thank you very much! Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet