Merced, We run 700 to 800 slides per day plus specials and IHC's. We use to wipe down but with staff and workload it was impossible to continue. Thanks for the input and the remember. Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-422-7907
>>> Merced M Leiker <lei...@buffalo.edu> 03/24/2010 3:33 PM >>> I use VWR VistaVision coverslips (for fluorescent imaging) and had noticed some dust/debris on them that would autofluoresce and give false positives with some of my stains. The problem disappeared when I got into the habit wiping all my coverslips with EtOH before mounting...this may be cumbersome if you're doing a lot of slides, however. But it has worked really well. Regards, Merced --On Wednesday, March 24, 2010 4:24 PM -0400 Helen Fedor <hfe...@jhmi.edu> wrote: > Hello, Our lab does IHC staining in house and have had zero problems with > our Fisher brand cover slips until recently. We've tried both Fisher and > Corning cover slips and what appears to be dust or imperfections in the > glass are found on both brands. This is creating false positives for us, > because when the positive staining is low it is difficult to impossible > to tell the imperfections from the staining itself. > > Any advice will be welcome. > > -- > Helen > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet