Hi Joe,
Thanks for that notice about flow rates. But I think for the mouse you
meant 1-3mls/min (not per 10min?)...
Regards,
Merced
--On Saturday, March 27, 2010 5:03 PM -0700 Joseph Saby
<saby_josep...@yahoo.com> wrote:
All-
From previous work with rat perfusions, the flow rate was about 10
ml/minute. If I had to guess, the equivalent flow rate for a mouse would
be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will
definitely cause blowout artefacts.
Joe Saby, BA HT
__________________________________________________
From: Merced M Leiker <lei...@buffalo.edu>
To: charles.scou...@leica-microsystems.com; mak...@ufl.edu;
histonet@lists.utsouthwestern.edu
Sent: Fri, March 19, 2010 9:21:38 AM
Subject: RE: [Histonet] mouse perfusion rate
The vasculature will leak too much and the mouse will get bloated -
you'll
see it first in either the intestines blowing up like a balloon or fluid
coming out of the nose. Just not the same as the heart pumping when the
mouse is alive with intact physiology and normal functioning. Don't know
exactly why, but that's what happens when you go too fast. Perhaps the
vasculature has lost its control to compensate for the pressure? I'm not
a
physiologist so I'm not sure why...maybe someone on the Histonet can
answer
that?
Regards,
Merced
--On Thursday, March 18, 2010 5:49 PM -0500
charles.scou...@leica-microsystems.com wrote:
Why not? What happens? One would think the mammalian cardiovascular
system could withstand physiological pressures and flow rates, at least
for one lifetime?
Cordially,
Charles W. Scouten, Ph.D
Product Manager, MNL
Biosystems Division
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL 60071
United States of America
Telephone 630 964 0501
facsimile +1 630 964 0576
www.MyNeuroLab.com
www.leica-microsystems.com
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From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M
Leiker <lei...@buffalo.edu>
Sent: Thursday, March 18, 2010 12:38 PM
To: MKing <mak...@ufl.edu>; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mouse perfusion rate
That may be mouse cardiac output, but I can assure you, from experience,
you do not want to perfuse at 17ml/min.
Regards,
Merced
--On Thursday, March 18, 2010 1:32 PM -0400 MKing < mak...@ufl.edu>
wrote:
Li,
Mouse cardiac output seems to be about 17 ml/min (e.g.
www.transonic.com/mice1.shtml), you probably want to try for that to
keep pressures close to physiological.
A syringe pump is pretty inexpensive and probably all you need.
Mike
----- Original Message -----
From: Li Zhang < dancingw...@yahoo.com>
Date: Wednesday, March 17, 2010 14:59
Subject: [Histonet] question about mouse perfusion
To: histonet@lists.utsouthwestern.edu
> > My question is: can anyone give me a rough idea of how fast I
> > should inject ( like ml/min). I think I've tried like 30 ml in 3
> > min, and I suspect that it's too fast because I do observe
> > tissue swelling sometimes.
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348 Biomedical Research Building
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lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
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Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
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lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
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