This question if for anyone doing C4d immunoflourescence staining. Could
someone share what protein concentration the Cy2 labeled anti-mouse IgG
is prepared at and what buffer is used for that? Our final dilution of
the Cy2 is (1:100 in 1%BSA/PBS) but we don't know what the original
protein concentration for the dry Cy2 should be. Thank you for your
assistance it is greatly appreciated.
Eric A. Breisch
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