Eric: Are you using Cy2 labeled goat anti-mouse IgG (H&L) from Jackson ImmunoResearch Labs? According to their catalog (product number 115-225-003), the stock is 2.0 mg/ml. At 1:100, your working concentration would be 20 ug/ml.
Jim B "Breisch, Eric" <ebrei...@rchsd.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 04/22/2010 03:51 PM To <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] protein concentration for Cy2 This question if for anyone doing C4d immunoflourescence staining. Could someone share what protein concentration the Cy2 labeled anti-mouse IgG is prepared at and what buffer is used for that? Our final dilution of the Cy2 is (1:100 in 1%BSA/PBS) but we don't know what the original protein concentration for the dry Cy2 should be. Thank you for your assistance it is greatly appreciated. Eric A. Breisch _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
