Or you could soak them in 10% sodium hydroxide for 20-30 minutes prior to processing. My techs say they cut like butter!
Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 North California Ave Chicago, IL 60625 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria Katleba Sent: Tuesday, April 27, 2010 5:22 PM To: zodia...@comcast.net; histonet@lists.utsouthwestern.edu Subject: [SPAM-HC] - RE: [Histonet] toe nails falling off slides - Email found in subject HOW TO CUT NAILS (or other difficult tissue) SO THAT THEY STAY on slide! (1) Cut the slides and place on Plus(+) slide.... (2) shake off excess water (3) place slide immediately in plastic Coplin jar with 1-3 drops of formalin at bottom. (4) place in hot oven for 5 mins (50-60 degrees) (5) remove from Coplin jar under a hood as formalin drops are now carcinogenic vapour (6) place slides in rack in oven so that the formalin moisture dries... (7) slide is ready for use Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net Sent: Tuesday, April 27, 2010 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] toe nails falling off slides To all, I was wondering if anyone out there had any tips on how to keep toenail sections from falling off slides. We mount them on positive slides, air dry for about an hour but they still fall off. Any insight on the subject would be greatly appreciated. Thanks, Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet