Give your pathologist a blind study and have them tell you which are microwave and which are conventional processed. This will tell you "where" the problem lies!
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, April 28, 2010 4:25 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave tissue We have just noticed an issue with our small biopsies lately. We are processing most of the small biopsies by a microwave processor. We finished our validation process about a couple of weeks ago and found little difference between the microwave processed tissue verses conventional processing. Now the pathologists are telling us that the nuclei do not seem as sharp and crisp as before. They of course think that the lack of sharpness is due to microwaving but obviously there are other issues to consider. We stain all of our biopsies on an automated stainer. Tomorrow we will again run some biopsies on the microwave and some on the conventional processor and see if there are any noticeable difference. Obviously if we see that the microwave tissue is not as crisp then we may need to adjust our microwave processing program. Another variable however was added about two weeks ago. We switched from Citrisolve to Clearite III on our stainer. Has anybody experienced similar issues with Clearite III on their automated stainer? Any other ideas? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
