Hi Andrew,
You must freeze fast to avoid the needle like crystals forming from the
water during freezing. Best - freeze in OCT in liquid Nitrogen.
It gets worse if you keep changing the temp of the block. For example keep
at -80 then bring to -20 cut than bring the temp down to remove the block
from the chuck and put back at -80. Few of those will make your tissue hard
to even recognize.
Good luck
Michelle HTL
USC Keck School of Medicine
LA, CA
----- Original Message -----
From: "Andrew Burgeson" <nap...@siscom.net>
To: <histonet@lists.utsouthwestern.edu>
Sent: Monday, May 03, 2010 12:31 PM
Subject: [Histonet] mouse kidney frozen sectioning
Having trouble with freezing artifact in the form of tiny
fissures or cracks in mouse kidney on frozen section.
Tissue is paraformaldehyde fixed and infiltrated w 70%
aqueous sucrose OCT solution.
Anyone else seen this and know how to deal with it?
Thx
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