Thanks for the tips everyone! I am going to order some agar (histogel) and use it! We are all very excited around here about this, we have been pulling our hair out over these darn pellets. Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA
________________________________ From: "Cormier, Kathleen" <kathleen.corm...@crl.com> To: Kim Merriam <kmerriam2...@yahoo.com> Sent: Wed, May 12, 2010 10:40:19 AM Subject: RE: [Histonet] (no subject) Hi Kim, Have you used Histogel? I have used it in the past (other places of employment) and on samples like yours. I just bought the gel, (not the whole unit, just the gel) scooped some out of the tube, placed into a weight boat, microwaves for a few seconds until liquid, then took a transfer pipet and dropped onto the cell pellet. I then placed the tube/pellet on a cold plate or fridge and wait until solid. I then popped out the now solid pellet, and processed as usual (no baggie/paper) in a cassette. Works like a charm.... Kathy Kathy Cormier Histology Manager Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6803 Fax: 978-988-8793 kathleen.corm...@crl.com The Charles River 24th Annual Short Course on Laboratory Animal Science will be held June 21-24, 2010, in Newton, MA. Click Here to get more information on this event. Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, May 12, 2010 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Good morning, I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot of cells along the way (especially when trying to take the cells out of the tube). We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again. At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing. I am losing a lot of the cells during this step, because the pellet is not quite solid. Do you think it would be OK to let the cells air-dry a bit and then take them out for processing? I know this goes against everything I was taught in histology, but I am really at a loss here. Anyone have any hot tips for me? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet </PRE><HR><font size="2" face="Arial">This email, its contents and attachments are confidential and may be covered by legal privilege. This email contains information intended only for the person(s) and/or entity named above. The views and opinions expressed are those of the sender and not necessarily those of Charles River Laboratories or its affiliates. Any other distribution, copying, review, use or disclosure is strictly prohibited. If you are not the intended recipient, please delete this message and any attachments without making a copy and advise the sender by return email - thank you. <BR></BR>This email and any attachments have been scanned for viruses prior to leaving the Charles River Laboratories network. Charles River Laboratories will not be liable for direct, special, indirect or consequential damages arising from alteration of the contents of this message by a third party or as a result of any virus being passed on. For more information on the range of services that we offer, please visit our website at <B><A href=http://www.criver.com>http://www.criver.com</A></B></font><PRE> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
