I know the histogel is pretty expensive (1500ish per kit), try ju= st
plain agar powder and mix with water...it is super cheap that way.
Happy spinning!! =)
Sarah Goebel, B.A., HT (ASCP)
H= istotechnician
XBiotech USA Inc.
8201 East Ri= verside Dr. Bldg 4 Suite 100
= Austin, Texas 78744<= /em>
-------- Original Message --------
Subject: Re: [Histonet] (no subject)
From: Kim Merriam <kmerriam2...@yahoo.com>
Date: Wed, May 12, 2010 7:49 am
To: "Cormier, Kathleen" <kathleen.corm...@crl.com>
Cc: Histonet <histonet@lists.utsouthwestern.edu>, sgoe...@xbiotech.co m
Thanks for the tips everyone! I am going to order some aga= r
(histogel) and use it! We are all very excited around here abo= ut
this, we have been pulling our hair out over these darn pellets.
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
<= br>
_________________________________________________________________
From: "Cormier, Kathleen" <kathleen.corm...@cr= l.com>
To: Kim Merri= am <kmerriam2...@yahoo.com>
= Sent: Wed, May 12, 2010 10:40:19 AM
Subject: RE: [Histonet] (no subject)
= Hi Kim,
Have you used Histogel? I have used it in the past (other pl= aces of
employment) and on samples like yours. I just bought the gel, (not the
whole unit, just the gel) scooped some out of the tube, placed
into a w= eight boat, microwaves for a few seconds until liquid, then
took a transfer= pipet and dropped onto the cell pellet. I then placed
the tube/pellet on a= cold plate or fridge and wait until solid. I
then popped out the now solid= pellet, and processed as usual (no
baggie/paper) in a cassette. Works like= a charm....
Kathy
Kathy Cormier
Histology Manager
Charles River Laboratories
251 Ballardvale Street
Wilmin= gton, MA 01887
Ph: 781-222-6803
Fax: 978-988-8793
[1]kathleen.corm...@crl.com
The = Charles River 24th Annual Short Course on Laboratory Animal
Science will be= held June 21-24, 2010, in Newton, MA. Click Here to
get more informa= tion on this event.
Accelerating Drug Development. Exactly.
N= otice - This email and any files transmitted with it are
confidential and m= ay contain privileged and/or proprietary
information. You must not disclose= this message to another party
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-----Orig= inal Message-----
From: [2]histonet-boun...@lists.utsouthwestern.edu
[mailto:[3]histonet-bounc= e...@lists.utsouthwestern.edu] On Behalf Of
Kim Merriam
Sent: Wednesda= y, May 12, 2010 9:24 AM
To: [4]histo...@lists.utsouthwestern.edu
Subject: [Histonet] (no subje= ct)
Good morning,
I am trying to make some nice FFPE cel= l pellet blocks, but I seem to
lose a lot of cells along the way (espe= cially when trying to take
the cells out of the tube). We are fixing = the cells in NBF,
spinning them down, adding 70% and spinning down again.&n= bsp; At
that point, I am trying to scoop out the cells (with a weighing sco op)
and wrap them in lens paper for processing. I am losing a lot of
= the cells during this step, because the pellet is not quite solid.
Do= you think it would be OK to let the cells air-dry a bit and then
take them= out for processing? I know this goes against everything I
was taught= in histology, but I am really at a loss here.
Anyone have any hot t= ips for me?
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge,= MA
__________________________________= _____________
Histonet mailing list
[5]histo...@lists.utsouthwestern.edu
[6]http://lists.utsouthwes= tern.edu/mailman/listinfo/histonet
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References
1. 3D"mailto:kathleen.corm...@crl.com";
2. 3D"mailto:histonet-bou 3.
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4. 3D"mailto:histonet 5. file://localhost/tmp/3D"mail 6. 3D"http://=/
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