I have found this helps..... 1. Embed the tissue in a dep mould, as this provides more stability, then 2. Face the block 3.. leave in -20 deg freezer overnight 4. remove from freezer and cut sections 5. If you have multiple blocks to work with, leave them in the freezer until ready to cut
regards On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia <reuel.corne...@tsrh.org>wrote: > How do you process a fibrous bone tissue ( 7 mm thick). We have use > Paraffin Type 9 from Richard allan Scientific to embed works well with our > bone femur( 7 mm) when cutting but on fibrous bone it does not give us a > good result in cutting the blocks. It is like cutting a uterus tissue but a > little bit harder. Please give me your opinion on how to remedy this kind of > tissue not mentioning double embedding method or plastic. Thank you. > > Reuel Cornelia > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet