Louise has very good advice here as related to paraffin processing of
this tissue. I may even add to soak the block a little more before
taking the final sections. However, have you ever thought of
processing into MMA resin? If you have these capabilities you may be
very pleased with the results and find the microtomy less problematic.
Let me know if or how I can be of assistance!
Jack
On May 15, 2010, at 4:30 AM, louise renton <louise.ren...@gmail.com>
wrote:
I have found this helps.....
1. Embed the tissue in a dep mould, as this provides more
stability, then
2. Face the block
3.. leave in -20 deg freezer overnight
4. remove from freezer and cut sections
5. If you have multiple blocks to work with, leave them in the
freezer until
ready to cut
regards
On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia <reuel.corne...@tsrh.org
>wrote:
How do you process a fibrous bone tissue ( 7 mm thick). We have use
Paraffin Type 9 from Richard allan Scientific to embed works well
with our
bone femur( 7 mm) when cutting but on fibrous bone it does not give
us a
good result in cutting the blocks. It is like cutting a uterus
tissue but a
little bit harder. Please give me your opinion on how to remedy
this kind of
tissue not mentioning double embedding method or plastic. Thank you.
Reuel Cornelia
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Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
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