Erin - Try this trick when using the freezing spray: take a folded kim wipe tissue and fold it again, then again then the long fold is folded twice so you end up with roughly a 1 inch by 1 inch multi layer slide wiper. Run your finger down each crease and after the final fold, give it a little pinch and twist so it holds its shape. You are probably wondering what does this have to do with freezing spray artifact... Dip the square of Kim Wipe in your water bath and press it on the cut surface of your tissue block. It will act as an insulator when you spray the block surface. They are also good for soaking the face of a block and are better than a wad of tissue when wiping slides.
JB "Martin, Erin" <erin.mar...@ucsf.edu> Sent by: histonet-boun...@lists.utsouthwestern.edu 05/19/2010 12:41 PM To histonet <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Freezing spray artifact Has anyone run into a problem or artifact from freezing spray? I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like. Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet