Sounds like you might want Metanil Yellow. I have found it doesn't fade as much if you let your slides dry a little before you dehydrate and coverslip.
Kim Donadio Pathology Supervisor Baptist Hospital 1000 W Moreno St. Pensacola FL 32501 Phone (850) 469-7718 Fax (850) 434-4996 "Mike Tighe" <mti...@trudeauinstitute.org> Sent by: histonet-boun...@lists.utsouthwestern.edu 05/24/2010 11:13 AM To <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] Yellow counterstain I have seen Tissue sections counterstained with a yellow counterstain. I would like to use an Iron hematoxylin with this counter stain. Can anyone tell me what this is? Are there any tips/tricks to this counterstain? Thanks in advance! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet