1% Periodic Acid Solution 0.5% Sodium Metabisulfite Periodic Acid....1 gm Sodium metabisulfite.0.5 gm Distilled water..100 ml Distilled water....100 ml
.1N HCL 3% Glacial Acetic Acid Conc. (10N) HCL.1 ml Conc. Glacial Acetic Acid..3 ml Distilled Water.100 ml Distilled water......100 ml Alcian Blue Solution pH 1.0 Alcian Blue Solution pH 2.5 Alcian Blue 8GX...1.0 gm Alcian Blue 8GX....1.0 gm 0.1N HCL....100 ml 3% Glacial Acetic Acid.100 ml Adjust pH to 2.5. Add a few Schiff's Reagent Thymol crystals for preservative. Purchased Commercially (ready to use) PROCEDURAL NOTES 1. pH 2.5 is routinely used in our laboratory. Use pH 1.0 only if the pathologist specifically requests you to do so. 2. Filter Alcian Blue solutions before use. 3. Use an aliquot from the stock bottle of Schiff's reagent. This may be reused but do not return to stock bottle. Change frequently and discard if solution is pink. PROCEDURE 1. Deparaffinize and hydrate to distilled water. 2. Place slides in freshly filtered Alcian Blue solution for 30 minutes. 3. If using Alcian Blue solution 2.5 - Wash in running water for 5 minutes. If using Alcian Blue solution 1.0 - Blot section dry with fine filter paper. 4. Oxidize in Periodic Acid solution for 10 minutes. 5. Wash in running water for 5 minutes. 6. Place slides in Schiff's reagent for 10 minutes. 7. Rinse in Sodium Metabisulfite solution, 3 changes; 2 minutes each. 8. Wash in running water for 10 minutes. 9. Dehydrate in 95% alcohol, 100% alcohol, clear in xylene, two changes each. 10. Coverslip. RESULTS 1. pH 2.5 (acid group) -Exclusively acid substances (various connective tissue mucins) - blue -Neutral polysaccharides (glycogen) - magenta -Both Alcian blue and PAS, yielding varying shades of purple to deep blue, color most epithelial mucins and cartilage ground substance. -Cell bodies of fungi - red to purple -Mucoid capsules - blue -Other features resemble PAS stain 2. pH 1.0 (sulphate group) Sulfated mucosubstances - blue REFERENCE 1. Lev, R., Spicer, S.S.;J. Histochem. Cystochem. 12:309,1964. Copyright by Williams and Wilkins Co. 2. C.F.A. Culling; Handbook of Histopathological and Histochemical Techniques, 3rh e. 1974. Butterworth. 3. Preece, Ann: Manual for Histologic Technicians 2nd ed. 1965. 4. AFIP Manual For Histologic Stain Methods, 3rd ed. 5. Schenk, E.A., M.D. and Mowry, R.W., MD>D, Journal of Histotechnology Vol. 6#2, June 1983. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ext 127 605-718-3779 (Fax) jmcgo...@clinlab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Diana McCaig Sent: Thursday, June 24, 2010 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AB-PAS for Barrett's Esophagus Can anyone share with me their procedure for AB-PAS for Barrett's Esophagus. With thanks Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet