Is anybody running the PIN 4 cocktail on Leica's Bond Max on human tissue? We are trying to bring PIN 4 into our lab. I can't seem to get consistent staining. I am using BioCare's PIN 4 antibodies, Mach 2 and Leica's RED Refine detection kit. I have used Leica's recommended times. We have a great positive control that worked wonderfully when first optimized, so I thought this Leica protocol was great. But could not duplicate results. The RED has always been fine. I have had BioCare replace their antibody.(nuclear staining very light, if there) Have changed protocols times as per BioCare's Tech Services. I am at my wits end. Help. What is your protocol? My director really wants this up and running. Thank you,
Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet