I have always soaked my biopsies on ice water. It always seems to help. Sent from my Verizon Wireless BlackBerry
-----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu Sender: histonet-boun...@lists.utsouthwestern.edu Date: Fri, 23 Jul 2010 13:01:46 To: <histonet@lists.utsouthwestern.edu> Reply-To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Laboratory Labeling Systems... (Barone, Carol ) 2. Re: Histonet Digest, Vol 80, Issue 26 (Stephanie Rodriguez) 3. Histology metrics (Scherer, Kris) 4. RE: HPV testing (McMahon, Loralee A) 5. RE: Biocare P63 with Dako Flex detection (Cynthia Pyse) 6. biohazard bags (Jeff Birkner) 7. RE: biohazard bags (Stacy McLaughlin) 8. Re: biohazard bags (dkb...@chs.net) 9. Sectioning problem (kgrob...@rci.rutgers.edu) 10. Re: Sectioning problem (Bryan Llewellyn) 11. Staining dish for vertical 30-slide rack (Morken, Tim) 12. RE: biohazard bags (Rathborne, Toni) 13. p-TBK-1 Ab (Michelle Mathews) 14. Artifacts in histology section (Aazath Raj) 15. Microwave Decalcification (Figliuolo, Leticia) 16. Re: Artifacts in histology section (Rene J Buesa) 17. artifacts of sectioning (Tench, Bill) 18. Formalin substitutes (Weaver, Colin) 19. RE: artifacts of sectioning (Matt Brooks) 20. HPV (rick.garnh...@memorialhealthsystem.com) ---------------------------------------------------------------------- Message: 1 Date: Thu, 22 Jul 2010 13:21:26 -0400 From: "Barone, Carol " <cbar...@nemours.org> Subject: [Histonet] Laboratory Labeling Systems... To: <histonet@lists.utsouthwestern.edu> Message-ID: <37e4bac017f57141af64faa5aeb04ce8033a7...@wlmmsx01.nemours.org> Content-Type: text/plain; charset="us-ascii" Histonetters....What are your thoughts: We are getting ready to purchase a labeling system for our Core Facility. We have limited $'s and have demo'ed three systems. We have many different projects,with varying numbers of samples, blocks and slides. We would like to cover the widest amount of needs, with the system....not just cassettes and slides, but samples in freezers to antibodies we have alloquotted. We are about to purchase a two-D bar-coding system that can label everything we need and work with our present EXCEL data bases. We are leaning toward a Brady System, where we can purchase individually the parts we need for labeling cassettes to eppy's and we especially like that they... not only have a label that can be attached to frozen vials... but that there are several types of scanners available for the different stations in our lab. This system meets our CER requirements and is up-gradable for adding more stations if needed at alater time. Sounds too good to be true....so that is where you all come in. Is anyone using a Brady Labeling System? Good? Bad? Problems? What are your thoughts? Thanks for the impromptu survey. We plan to order in August, if there are no "big" issues, with those that use it. We demo's the labels and are happy with them. We are going alpha numeric/2-D...3 lines of text, is needed for our research work. Thanks for your thoughts. C. Barone Director Histotechnology Core Lab cbar...@nemours.org ------------------------------ Message: 2 Date: Thu, 22 Jul 2010 10:44:10 -0700 From: Stephanie Rodriguez <srodrig...@phenopath.com> Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 26 To: <histonet@lists.utsouthwestern.edu> Message-ID: <c86dd17a.d08%srodrig...@phenopath.com> Content-Type: text/plain; charset="US-ASCII" As do we. Stephanie Rodriguez, HTL(ASCP), QIHC Senior Molecular Technologist-FISH IHC Technologist III Phenopath Laboratories Seattle, WA (206) 374-9000 On 7/22/10 10:04 AM, "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> wrote: > > Message: 4 > Date: Thu, 22 Jul 2010 08:18:27 -0700 > From: "Tench, Bill" <bill.te...@pph.org> > Subject: [Histonet] hpv testing on head and neck ca's > To: histonet@lists.utsouthwestern.edu > Message-ID: <2820431bf953bb4da3e9e1a5882265fd034a5...@mail1.pph.local> > Content-Type: text/plain; charset=us-ascii > > We do p16 on squamous ca of the head and neck region routinely > > Bill Tench > Associate Dir. Laboratory Services > Chief, Cytology Services > Palomar Medical Center > 555 E. Valley Parkway > Escondido, California 92025 > bill.te...@pph.org > Voice: 760- 739-3037 > Fax: 760-739-2604 This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 3 Date: Thu, 22 Jul 2010 13:43:52 -0400 From: "Scherer, Kris" <k...@idxpathology.com> Subject: [Histonet] Histology metrics To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <0c15e5fc826b334ba019e489846d02357e06c23...@rtwecs03.lca.labcorp.com> Content-Type: text/plain; charset="us-ascii" We are in need of a metrics for use in requesting an additional histotech. Does anyone have a system in place for presenting the need for an additional FTE, based on volume, worklow, vacations, etc? ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ------------------------------ Message: 4 Date: Thu, 22 Jul 2010 13:47:54 -0400 From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> Subject: RE: [Histonet] HPV testing To: Richard Cartun <rcar...@harthosp.org>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <c27aa2a01cef31469813089e226f582e02d03da...@urmcms7.urmc-sh.rochester.edu> Content-Type: text/plain; charset="us-ascii" Hi Dr. Cartun, We are running the HPV Genepoint test from Dako (in situ test). Along with the EGFR IHC. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun [rcar...@harthosp.org] Sent: Thursday, July 22, 2010 11:01 AM To: Histonet Subject: [Histonet] HPV testing What are labs doing for HPV testing on head and neck cancers when a request is received from a clinician? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 22 Jul 2010 15:10:24 -0400 From: "Cynthia Pyse" <cp...@x-celllab.com> Subject: RE: [Histonet] Biocare P63 with Dako Flex detection To: <cathy.crump...@tuality.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <000001cb29d1$89f157b0$9dd407...@com> Content-Type: text/plain; charset="us-ascii" Cathy Have you tried adding an EnVision FLEX mouse linker to your protocol. Sometimes that will work. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cathy.crump...@tuality.org Sent: Wednesday, July 21, 2010 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare P63 with Dako Flex detection Hi all, I have ran into a troubleshooting dilemma and was wonderi=g if anyone else could help. Is there anyone out there that uses the Biocare Medical P63 concentrated antibody with the Dako TRS and Flex detec=tion kit? I am curious what dilution you use and if you have to do an=ything special for the P63 (extra long retrieval, DAB enhancer, etc). = am having problems getting a strong signal for the basal cells in prosta=e. I am running it at 1:50 with 40 minutes in pH 9 retrieval (which =seems too long to me but does help). A dilution with the same protoco= at 1:100 is way too light. Thanks, <=DIV> Cathy Crumpton HT(ASCP), Histology Lead Tuality Community Hosp=tal Hillsboro, OR 97123 (503)681-1292 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 22 Jul 2010 14:11:18 -0500 From: "Jeff Birkner" <jbirk...@colabserv.com> Subject: [Histonet] biohazard bags To: <histonet@lists.utsouthwestern.edu> Message-ID: <efd452eeb5f9a04d8919c4228c5c392d04dc6...@p1ws0001.orhcnet.local> Content-Type: text/plain; charset="us-ascii" We are looking into our current use of biohazard bags. How many of you are currently re-using any bags for specimen transport? These would only be bags were the actual specimen was inside a secondary container and thus never directly came into contact with the bags. Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirk...@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. ------------------------------ Message: 7 Date: Thu, 22 Jul 2010 15:38:01 -0400 From: "Stacy McLaughlin" <stacy_mclaugh...@cooley-dickinson.org> Subject: RE: [Histonet] biohazard bags To: "Jeff Birkner" <jbirk...@colabserv.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <cb8866a9d4ecb049968db571cf4863a3060de...@cdhmail01.cooley-dickinson.org> Content-Type: text/plain; charset="iso-8859-1" Re-use of specimen transport bio-bags is a big NO-NO in my laboratory. You run the risk of accidentally using one that is contaminated. (This actually happened, and it was not a pretty sight.) You can't guarantee that the outside of that specimen container in the bag is not contaminated, even though it looks clean. You don't know the practices of the person that collected it. This could be a big liability and the possible consequences just aren't worth it. Just my 2 cents. Stacy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Jeff Birkner Sent: Thursday, July 22, 2010 3:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] biohazard bags We are looking into our current use of biohazard bags. How many of you are currently re-using any bags for specimen transport? These would only be bags were the actual specimen was inside a secondary container and thus never directly came into contact with the bags. Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirk...@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 22 Jul 2010 15:42:34 -0400 From: dkb...@chs.net Subject: Re: [Histonet] biohazard bags To: "Jeff Birkner" <jbirk...@colabserv.com> Cc: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Message-ID: <of57d1f229.06c9a70c-on85257768.006c1721-85257768.006c4...@chs.net> Content-Type: text/plain; charset="US-ASCII" We were doing just that and our Infection Control Nurse has banded this practice. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net "Jeff Birkner" <jbirk...@colabserv.com> Sent by: histonet-boun...@lists.utsouthwestern.edu 07/22/2010 03:11 PM To <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] biohazard bags We are looking into our current use of biohazard bags. How many of you are currently re-using any bags for specimen transport? These would only be bags were the actual specimen was inside a secondary container and thus never directly came into contact with the bags. Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirk...@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 9 Date: Thu, 22 Jul 2010 16:12:56 -0400 (EDT) From: kgrob...@rci.rutgers.edu Subject: [Histonet] Sectioning problem To: "histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <8b576f6ad1bfefb4659958e4c7c10cee.squir...@webmail.rci.rutgers.edu> Content-Type: text/plain;charset=iso-8859-1 I am working on an old microtome, a Reichert Histostat with a Tissue-Tek II microtome chuck adapter, and the last time I had this machine cleaned and rehabbed by my repairman, he said that the springs were shot on the chuck adapter, resulting in the windowblind chatter that I was getting in my sections. Unfortunately he did not have a replacement, Sakura doesn't make or sell them anymore, and the one source for replacement adapters I did find online sold me a part that had the same problem-shot springs (I assume this, as I was getting the same chatter with the "new" part). I have Googled, searched on eBay, checked online used scientific/medical equipment suppliers to no avail...but I will keep looking in case something comes up. So I guess my question is...would one of the newer cassette holders fit in my microtomes? In addition to the Reichert mentioned above, I also have an American Optical 860. Or is it possible to figure out how to replace the springs in the thing? (I have half a mind to wander over to the mechanical engineering dept. here to ask that question, but I figured I'd start here on Histonet.) I would really hate to have to replace the microtomes just because I can't get a cassette holder for them. Thanks for all your help, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 ------------------------------ Message: 10 Date: Thu, 22 Jul 2010 14:02:24 -0700 From: "Bryan Llewellyn" <llewl...@shaw.ca> Subject: Re: [Histonet] Sectioning problem To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <9cae5b0417c54c3d9f56ab57b4027...@bryanpc> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I had spring problems on the block holder of a microtome many years ago. I solved it by getting a local engineering shop to make me some new springs using the old springs as a template. It worked fine, and was cheap, too. Bryan Llewellyn ----- Original Message ----- From: <kgrob...@rci.rutgers.edu> To: "histonet" <histonet@lists.utsouthwestern.edu> Sent: Thursday, July 22, 2010 1:12 PM Subject: [Histonet] Sectioning problem I am working on an old microtome, a Reichert Histostat with a Tissue-Tek II microtome chuck adapter, and the last time I had this machine cleaned and rehabbed by my repairman, he said that the springs were shot on the chuck adapter, resulting in the windowblind chatter that I was getting in my sections. Unfortunately he did not have a replacement, Sakura doesn't make or sell them anymore, and the one source for replacement adapters I did find online sold me a part that had the same problem-shot springs (I assume this, as I was getting the same chatter with the "new" part). I have Googled, searched on eBay, checked online used scientific/medical equipment suppliers to no avail...but I will keep looking in case something comes up. So I guess my question is...would one of the newer cassette holders fit in my microtomes? In addition to the Reichert mentioned above, I also have an American Optical 860. Or is it possible to figure out how to replace the springs in the thing? (I have half a mind to wander over to the mechanical engineering dept. here to ask that question, but I figured I'd start here on Histonet.) I would really hate to have to replace the microtomes just because I can't get a cassette holder for them. Thanks for all your help, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (732) 445-6914 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 22 Jul 2010 15:40:45 -0700 From: "Morken, Tim" <timothy.mor...@ucsfmedctr.org> Subject: [Histonet] Staining dish for vertical 30-slide rack To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1aaf670737f193429070841c6b2add4c023d27b...@exmbmcb15.ucsfmedicalcenter.org> Content-Type: text/plain; charset=us-ascii Does anyone know of a plastic, inexpensive staining dish for the Richard-Allan or Raymond Lamb 30-slide vertical rack. Something similar to the tissue tek plastic stain dish. The Rack is 11cm / 4 3/8" long Tim Morken Supervisor, Histology / IPOX UCSF Medical Center Box 1656 1600 Divisadero St. San Francisco, CA 94143-1656 USA Phone: (415) 514-6042 Pager: (415) 443-6509 Fax: (415) 885-7409 Email: tim.mor...@ucsfmedctr.org<mailto:tim.mor...@ucsfmedctr.org> ------------------------------ Message: 12 Date: Fri, 23 Jul 2010 08:04:48 -0400 From: "Rathborne, Toni" <trathbo...@somerset-healthcare.com> Subject: RE: [Histonet] biohazard bags To: "Stacy McLaughlin" <stacy_mclaugh...@cooley-dickinson.org>, "Jeff Birkner" <jbirk...@colabserv.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <e78340c766a5284d999f5f5891ddf89014474...@smcmail.somerset-healthcare.com> Content-Type: text/plain; charset="utf-8" I agree. We never re-use them at my facility. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Stacy McLaughlin Sent: Thursday, July 22, 2010 3:38 PM To: Jeff Birkner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] biohazard bags Re-use of specimen transport bio-bags is a big NO-NO in my laboratory. You run the risk of accidentally using one that is contaminated. (This actually happened, and it was not a pretty sight.) You can't guarantee that the outside of that specimen container in the bag is not contaminated, even though it looks clean. You don't know the practices of the person that collected it. This could be a big liability and the possible consequences just aren't worth it. Just my 2 cents. Stacy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Jeff Birkner Sent: Thursday, July 22, 2010 3:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] biohazard bags We are looking into our current use of biohazard bags. How many of you are currently re-using any bags for specimen transport? These would only be bags were the actual specimen was inside a secondary container and thus never directly came into contact with the bags. Thanks! Jeffrey C. Birkner, CT(ASCP) Manager, Pathology Laboratory Section Collaborative Laboratory Services, L.L.C. 1005 Pennsylvania Ave, Suite 102 Ottumwa, IA 52501 641-455-5414 ORHC Extension #3538 jbirk...@colabserv.com The information contained in this communication may be confidential, is intended only for the use of the recipient(s) named above, and may be legally privileged. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ------------------------------ Message: 13 Date: Fri, 23 Jul 2010 07:15:58 -0700 (PDT) From: Michelle Mathews <mchelle252...@yahoo.com> Subject: [Histonet] p-TBK-1 Ab To: histonet@lists.utsouthwestern.edu Message-ID: <510051.54589...@web53308.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii Good morning everyone, I work in a research lab and am trying to optimize a p-TBK-1 antibody; I am running this work-up on a BondIII and a BondMax with a Dako Envision Rabbit kit for detection and secondary. I have only seen very faint staining and am wondering if anyone out there has worked with this and, if so, what type of control tissue would show the best signal. Thanks for any help, Michelle Mathews, HT(ASCP) TPL, UNC-CH ------------------------------ Message: 14 Date: Fri, 23 Jul 2010 20:56:28 +0530 From: Aazath Raj <aaz...@hotmail.com> Subject: [Histonet] Artifacts in histology section To: <histonet@lists.utsouthwestern.edu> Message-ID: <snt132-w57fcd77c239976388cfd03c8...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _________________________________________________________________ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/ ------------------------------ Message: 15 Date: Fri, 23 Jul 2010 11:34:59 -0400 From: "Figliuolo, Leticia" <leticia.figliu...@roche.com> Subject: [Histonet] Microwave Decalcification To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <069e6cf048b915488720412dafd1474d01ba5d9...@rnumsem702.nala.roche.com> Content-Type: text/plain; charset="us-ascii" Hello all, We inherited a Pelco Biowave 34700 (from Ted Pella) and would like to optimize decalcification of rats knee joints, dogs rib using the Biowave. I was wondering if anyone have a protocol for decalcification using nitric acid or formic acid or EDTA using microwave or if you know someone that uses microwave technology for decalcification that I can ask. Your help is appreciated. Leticia ------------------------------ Message: 16 Date: Fri, 23 Jul 2010 08:59:06 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Artifacts in histology section To: histonet@lists.utsouthwestern.edu, Aazath Raj <aaz...@hotmail.com> Message-ID: <902127.95344...@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Of the 3 probable causes, the most likely to be is the blade edge not uniformly sharp René J. --- On Fri, 7/23/10, Aazath Raj <aaz...@hotmail.com> wrote: From: Aazath Raj <aaz...@hotmail.com> Subject: [Histonet] Artifacts in histology section To: histonet@lists.utsouthwestern.edu Date: Friday, July 23, 2010, 11:26 AM Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section. with regards, Aazathraj.P Technical Officer, Apollo Hospitals-chennai India. aaz...@hotmail.com _________________________________________________________________ The latest in fashion and style in MSN Lifestyle http://lifestyle.in.msn.com/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Fri, 23 Jul 2010 09:09:52 -0700 From: "Tench, Bill" <bill.te...@pph.org> Subject: [Histonet] artifacts of sectioning To: histonet@lists.utsouthwestern.edu Message-ID: <2820431bf953bb4da3e9e1a5882265fd034a5...@mail1.pph.local> Content-Type: text/plain; charset=us-ascii It sounds like what you are describing is what we call "chatter." it seems to occur most frequently with GI biopsies, esp colon polyps and usually in the abnormal epithelium. My techs tell me that cooling the block better on the ice block makes it go away. i have no personal experience, other than to say that when it occurs, it makes the slide of very poor quality. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- ------------------------------ Message: 18 Date: Fri, 23 Jul 2010 17:15:05 +0100 From: "Weaver, Colin" <c.wea...@vla.defra.gsi.gov.uk> Subject: [Histonet] Formalin substitutes To: <histonet@lists.utsouthwestern.edu> Message-ID: <0a1c03fc4e63ee4aa24a7983e91c25b502938...@vla-exchn1.cvlnt.vla.gov.uk> Content-Type: text/plain; charset="us-ascii" Hi everyone - does anyone know how effective these formalin substitutes are at killing microorgansms9esp HG3) in the tissue fixed? I know that Finefix states that because of the alcohol content being over 70% that it is effective against many micro-organisms but info seems scarce on the others Thanks and have a nice weekend Colin Weaver Histology lab manager VLA Thirsk North Yorkshire UK </pre><br>Veterinary Laboratories Agency (VLA)<br><br>This email and any attachments is intended for the named<br>recipient only.<br>If you have received it in error you have no authority to use,<br>disclose, store or copy any of its contents and you should destroy<br>it and inform the sender. Whilst this email and associated attachments will have been<br>checked for known viruses whilst within VLA systems we can<br>accept no responsibility once it has left our systems.<br>Communications on VLA's computer systems may be monitored<br>and/or recorded to secure the effective operation of the system<br>and for other lawful purposes.<br><pre> ------------------------------ Message: 19 Date: Fri, 23 Jul 2010 09:20:34 -0700 From: "Matt Brooks" <mbro...@incytepathology.com> Subject: RE: [Histonet] artifacts of sectioning To: <histonet@lists.utsouthwestern.edu> Message-ID: <706224670091fe47997aef88efade7ca01550...@exchange-srv.pai.e-pathology.com> Content-Type: text/plain; charset="us-ascii" What I have used in the past is a short warm water soak, 10-15 seconds, in the water bath, after facing in and between sections, then placing the block back on ice. I would use a small 200 mL beaker filled with DI water sitting inside the water, it helps reduce junk floating the bath. Be careful to avoid over soaking. Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Friday, July 23, 2010 9:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] artifacts of sectioning It sounds like what you are describing is what we call "chatter." it seems to occur most frequently with GI biopsies, esp colon polyps and usually in the abnormal epithelium. My techs tell me that cooling the block better on the ice block makes it go away. i have no personal experience, other than to say that when it occurs, it makes the slide of very poor quality. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Fri, 23 Jul 2010 10:25:29 -0600 From: rick.garnh...@memorialhealthsystem.com Subject: [Histonet] HPV To: histonet@lists.utsouthwestern.edu Message-ID: <ofec5d0e64.22985806-on87257769.005a2e9f-87257769.005a3...@memorialhealthsystem.com> Content-Type: text/plain; charset=US-ASCII We are doing the P16 antibody from MTM Labs. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 27 ****************************************
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