I have used a phospho-H3 Ser10 antibody successfully on FFPE sections of adult mouse heart and mouse embryos. Ours is Millipore 06-570 (formerly Upstate 06-570) Lot# DAM1644557 and HIER with 10mM sodium citrate buffer, 0.05% Tween-20 pH 6.0 works great.
In the past we tried a phospho H3 Ser10 antibody from Cell Signaling Technology #9706 and it works fine on Western blots, but we could never get it to work on immunostainings. This is a different antibody and company from Millipore/Upstate. Andrea Marion Graduate Student University of Illinois - Chicago amario3 [at] uic [dot] edu I have been trying without success to get this antibody to work. Is anyone doing this antibody? We have the Leica Bond Max and would like to perform it on this platform. I have tried 2 antibodies from Cell Signaling Technology without any success. Any advice would be greatly appreciated. Thanks! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet