Try reducing the processing time and try very fine cutting and I think the microchatter will go.
Thanks Prashant Sareen BS , M.B.A , HT (ASCP) Associate Scientist III Comprehensive Animal health Services Bioreliance Corporation 14930 Broschart Road, Rockville, MD 20850 Office: 301-610-2744 Histology: 301-610-2997 Cell: 240-315-5541 Email: prashant.sar...@bioreliance.com > From: histonet-requ...@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 80, Issue 30 > To: histonet@lists.utsouthwestern.edu > Date: Mon, 26 Jul 2010 10:02:53 -0700 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Artifacts in histology section (Joseph Saby) > 2. Re water problem (Steven Weston) > 3. RE: Re water problem (Goins, Tresa) > 4. Re: Artifacts in histology section (kim.dona...@bhcpns.org) > 5. Special Stain Storage (kristen arvidson) > 6. RE: Special Stain Storage (Podawiltz, Thomas) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 25 Jul 2010 10:49:05 -0700 (PDT) > From: Joseph Saby <saby_josep...@yahoo.com> > Subject: Re: [Histonet] Artifacts in histology section > To: Aazath Raj <aaz...@hotmail.com>, histonet@lists.utsouthwestern.edu > Message-ID: <431113.5115...@web114420.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > What you are describing might be microchatter. These will be sharp parallel > lines/cracks that run parallel to the knife edge and are only visible under > the > microscope. > > The usuall cause is a combination of overprocessing and rough facing that is > too > aggressive and/or with too dull a blade. Overprocessing makes the tissue > very > hard and somewhat brittle. The thick sections/dull knife cause the tissue to > compress and then release, causing the chatter. The actual danage is in the > block face. > > Once you have the problem in a block, if the tissue is thick enough, you > might > be able to repeatedly soak the block in ice water and gently (with a fairly > sharp knife) reface. With luck, you might be able to get through the damaged > block face. > > > Another artifact I have seen is similar, but the chatter appears very blurry. > > This is usually caused be poor fixation/processing, then oversoaking the > blocks > after facing. The trick here is to reface the block, then chill it without > exposure to water. I've sectioned such blocks after placing them in a > freezer > to chill them thoroughly. This will help to obtain a section, but may not > fix > the staining problems that might show up later. > > > Good luck! > > Joe Saby, BA HT > > > > > ________________________________ > From: Aazath Raj <aaz...@hotmail.com> > To: histonet@lists.utsouthwestern.edu > Sent: Fri, July 23, 2010 11:26:28 AM > Subject: [Histonet] Artifacts in histology section > > > > Dear Friends, > > I am an Histology Technologist. I am having a problem > here,while > sectioning am not seeing and scoring artifacts on the section but in the > microscope am seeing a tearing artifacts particularly in endoscopy biopsies. > am > not able to locate where is the problem,is that because of blades or due to > micro-crystallization of wax or due to any processing problem. Its not > consistently in all but i get it on some blocks every day. Can any one help > me > in sorting it out. If anybody is interested in will send the picture of those > section. > > > > > > with regards, > > Aazathraj.P > > Technical Officer, > > Apollo Hospitals-chennai > > India. > > aaz...@hotmail.com > > > > _________________________________________________________________ > The latest in fashion and style in MSN Lifestyle > http://lifestyle.in.msn.com/_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 2 > Date: Mon, 26 Jul 2010 13:49:31 +1000 > From: Steven Weston <steven.wes...@utas.edu.au> > Subject: [Histonet] Re water problem > To: histonet list <histonet@lists.utsouthwestern.edu> > Message-ID: <c87342eb.2535%steven.wes...@utas.edu.au> > Content-Type: text/plain; charset="iso-8859-1" > > We had this problem when we started using APTS coated slides and left out > adhesive from the water bath. A simple solution I have found is to add a > single drop of triton x100 (or similar detergent) to my full water bath and > mix before heating the water. This reduces the surface tension of the water > and allows it to run off the slide. > Regards > Steve weston > Menzies Research Institute > Hobart Tasmania, Australia > > > ------------------------------ > > Message: 3 > Date: Mon, 26 Jul 2010 15:39:05 +0000 > From: "Goins, Tresa" <tgo...@mt.gov> > Subject: [Histonet] RE: Re water problem > To: Steven Weston <steven.wes...@utas.edu.au> > Cc: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <ca4df32ed505d94bb55e95487d8e9841049...@doaisd5205.state.mt.ads> > Content-Type: text/plain; charset="us-ascii" > > Thanks for the great tip Steve - it works great! > > > Tresa Goins > Veterinary Diagnostic Lab > Department of Livestock > Bozeman, Montana > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Steven Weston > Sent: Sunday, July 25, 2010 9:50 PM > To: histonet list > Subject: [Histonet] Re water problem > > We had this problem when we started using APTS coated slides and left out > adhesive from the water bath. A simple solution I have found is to add a > single drop of triton x100 (or similar detergent) to my full water bath and > mix before heating the water. This reduces the surface tension of the water > and allows it to run off the slide. > Regards > Steve weston > Menzies Research Institute > Hobart Tasmania, Australia > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Mon, 26 Jul 2010 10:57:58 -0500 > From: kim.dona...@bhcpns.org > Subject: Re: [Histonet] Artifacts in histology section > To: Joseph Saby <saby_josep...@yahoo.com> > Cc: histonet@lists.utsouthwestern.edu, Aazath Raj > <aaz...@hotmail.com>, histonet-boun...@lists.utsouthwestern.edu > Message-ID: > <off4b67742.f26d1a0c-on8625776c.0057150c-8625776c.0057b...@bhcpns.org> > Content-Type: text/plain; charset="US-ASCII" > > It's very difficult to diagnosis a problem such as this without hands on. > But one thing that I noticed years ago that was so simple could cause > this. A build up of tissues on the back of the blade holder. Worth a look. > > > > > > Kim Donadio > Pathology Supervisor > Baptist Hospital > 1000 W Moreno St. > Pensacola FL 32501 > Phone (850) 469-7718 > Fax (850) 434-4996 > > > > Joseph Saby <saby_josep...@yahoo.com> > Sent by: histonet-boun...@lists.utsouthwestern.edu > 07/25/2010 12:49 PM > > To > Aazath Raj <aaz...@hotmail.com>, histonet@lists.utsouthwestern.edu > cc > > Subject > Re: [Histonet] Artifacts in histology section > > > > > > > What you are describing might be microchatter. These will be sharp > parallel > lines/cracks that run parallel to the knife edge and are only visible > under the > microscope. > > The usuall cause is a combination of overprocessing and rough facing that > is too > aggressive and/or with too dull a blade. Overprocessing makes the tissue > very > hard and somewhat brittle. The thick sections/dull knife cause the tissue > to > compress and then release, causing the chatter. The actual danage is in > the > block face. > > Once you have the problem in a block, if the tissue is thick enough, you > might > be able to repeatedly soak the block in ice water and gently (with a > fairly > sharp knife) reface. With luck, you might be able to get through the > damaged > block face. > > > Another artifact I have seen is similar, but the chatter appears very > blurry. > This is usually caused be poor fixation/processing, then oversoaking the > blocks > after facing. The trick here is to reface the block, then chill it > without > exposure to water. I've sectioned such blocks after placing them in a > freezer > to chill them thoroughly. This will help to obtain a section, but may not > fix > the staining problems that might show up later. > > > Good luck! > > Joe Saby, BA HT > > > > > ________________________________ > From: Aazath Raj <aaz...@hotmail.com> > To: histonet@lists.utsouthwestern.edu > Sent: Fri, July 23, 2010 11:26:28 AM > Subject: [Histonet] Artifacts in histology section > > > > Dear Friends, > > I am an Histology Technologist. I am having a problem > here,while > sectioning am not seeing and scoring artifacts on the section but in the > microscope am seeing a tearing artifacts particularly in endoscopy > biopsies. am > not able to locate where is the problem,is that because of blades or due > to > micro-crystallization of wax or due to any processing problem. Its not > consistently in all but i get it on some blocks every day. Can any one > help me > in sorting it out. If anybody is interested in will send the picture of > those > section. > > > > > > with regards, > > Aazathraj.P > > Technical Officer, > > Apollo Hospitals-chennai > > India. > > aaz...@hotmail.com > > > > _________________________________________________________________ > The latest in fashion and style in MSN Lifestyle > http://lifestyle.in.msn.com/_______________________________________________ > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ----------------------------------------- > All electronic data transmissions originating from or sent to > Baptist Health Care Corporation (BHC) are subject to monitoring. > This message along with any attached data, are the confidential and > proprietary communications of BHC and are intended to be received > only by the individual or individuals to whom the message has been > addressed. If the reader of this message is not the intended > recipient, please take notice that any use, copying, printing, > forwarding or distribution of this message, in any form, is > strictly prohibited and may violate State or Federal Law. If you > have received this transmission in error, please delete or destroy > all copies of this message. For questions, contact the BHC Privacy > Officer at (850) 434-4472. Rev.10/07. > > ------------------------------ > > Message: 5 > Date: Mon, 26 Jul 2010 09:07:57 -0700 (PDT) > From: kristen arvidson <arvidsonkris...@yahoo.com> > Subject: [Histonet] Special Stain Storage > To: histonet <histonet@lists.utsouthwestern.edu> > Message-ID: <820784.89949...@web65707.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello All, > We were recently inspected by CLIA and our inspector noticed that we didn't > have reagent storage temperatures written in our Special Stains procedures. > We do our stains by hand so we do have some stored in the refrigerator and > others stored at room temp. I went through some of the Histo books and I > cannot find any specifics on storage of reagents. Any suggestions?? > > Thanks!! > Kristen > > > > > ------------------------------ > > Message: 6 > Date: Mon, 26 Jul 2010 12:53:38 -0400 > From: "Podawiltz, Thomas" <tpodawi...@lrgh.org> > Subject: RE: [Histonet] Special Stain Storage > To: 'kristen arvidson' <arvidsonkris...@yahoo.com>, histonet > <histonet@lists.utsouthwestern.edu> > Message-ID: > <38667e7fb77ecd4e91bfaeb8d986386323d5df8...@lrghexvs1.practice.lrgh.org> > > Content-Type: text/plain; charset="iso-8859-1" > > That is a new one on me. It has never come up during any of our inspections. > > > Tom Podawiltz HT (ASCP) > Histology Section Head/Laboratory Safety Officer > LRGHealthcare > > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen > arvidson > Sent: Monday, July 26, 2010 12:08 PM > To: histonet > Subject: [Histonet] Special Stain Storage > > Hello All, > We were recently inspected by CLIA and our inspector noticed that we didn't > have reagent storage temperatures written in our Special Stains procedures. > We do our stains by hand so we do have some stored in the refrigerator and > others stored at room temp. I went through some of the Histo books and I > cannot find any specifics on storage of reagents. Any suggestions?? > > Thanks!! > Kristen > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential > information intended only for the use of the recipient(s) named above. If you > are not the intended recipient, you may not print,distribute, or copy this > message or any attachments. If you have received this communication in error, > please notify the sender by return e-mail and delete this message and any > attachments from your computer. Any views or opinions expressed are solely > those of the author and do not necessarily represent those of LRGHealthcare. > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 80, Issue 30 > **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
