I have used formical for years and it works great. You don'= t have to pre-fix your samples because there is formalin in with the decal. It fixes and decals at the same time. I don't know how many sp ecimens you have, but a sure fire way to check is to weigh the bone after g= rossing. Then everyday weigh it and replace the decal solution. = It should start losing weight because the calcium is coming out. As = soon as the sample starts gaining weight, take it out. Now it is just= absorbing fluid. In the past when I have done huge bones, I use this= technique (learned at a NSH convention) and it works like a charm! H= ope that helps =)
PS-The formical is made by Dec= al company. Sarah Goebel, B.A., HT (ASCP) Histotechnician <= /span> XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 = Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] decalcification From: "Webb, Dorothy L" <[1]dorothy.l.w...@healthpartners.com> Date: Mon, August 02, 2010 7:14 am To: "'[2]histo...@lists.u= tsouthwestern.edu'" <[3]histo...@lists.uts= outhwestern.edu> I would appreciate any feedback on what all are using in your decalcificati= on process. We get a lot of large bones in and the past 2-3 months have no= ticed a huge problem in our microtomy process with these samples. We have = been grossing the bones in and leaving the sample in the cassette in 10% fo= rmalin for 24 hours befoere placing in decal for up to 8 hours and still ha= ving the inner portion of the sample look underprocessed and crunchy! Any = suggestions would be appreciated! Dorothy Webb, HT Regions Hospital Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are inte= nded solely for the use of the individual or entity to whom they are addres= sed. If you are not the intended recipient or the individual responsible fo= r delivering the e-mail to the intended recipient, please be advised that y= ou have received this e-mail in error and that any use, dissemination, forw= arding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the He= althPartners Support Center by telephone at (952) 967-6600. You will be rei= mbursed for reasonable costs incurred in notifying us. HealthPartners R001.= 0 _______________________________________________ Histonet mailing list [4]histo...@lists.utsouth= western.edu [5]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:dorothy.l.w...@healthpartners 2. 3D"mailto:histonet@lists.utsouthwestern.edu"; 3. 3D"mailto:histonet@lists.utsouthwestern.edu"; 4. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
