You cannot set a fixed time for decalcification. You have to leave the bone in the decalcifying solution until iy is soft and ready for processing. René J.
--- On Mon, 8/2/10, Webb, Dorothy L <dorothy.l.w...@healthpartners.com> wrote: From: Webb, Dorothy L <dorothy.l.w...@healthpartners.com> Subject: [Histonet] decalcification To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Date: Monday, August 2, 2010, 10:14 AM I would appreciate any feedback on what all are using in your decalcification process. We get a lot of large bones in and the past 2-3 months have noticed a huge problem in our microtomy process with these samples. We have been grossing the bones in and leaving the sample in the cassette in 10% formalin for 24 hours befoere placing in decal for up to 8 hours and still having the inner portion of the sample look underprocessed and crunchy! Any suggestions would be appreciated! Dorothy Webb, HT Regions Hospital Technical Supervisor 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
