I am wondering how much agitation is required in order to achieve the clearing of paraffin from the tissue and slide. IHC systems obviously use adhesive or + slides, aiding in tissue adherence. Too much agitation might take take tissue off. And what about nail fragments or hard tissues in general? Will they survive? I have an article and info regarding this type of deparaffinization and will try to get it into an e-mail.
My sense is that it could very well be less efficient and time consuming and that depending on how it is done, could yield very different results. I still think that "there's nothing like xylene!!!" But obviously soap and water doesnt hurt your liver.... Interesting method I would like to hear more about people's experiences. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
