It depends on the number of slides as well as time on the shelf and a little common sense.Your stock solutions have a longer shelf life than your working. I worked in a lab that perfomed a large number of Masson's by hand( until purchasing the Artisan) We changed the solutions once a week to insure consistent staining. If you perform these stains only twice a month, your results will be more consistant if you make the Weigert's fresh and watch the level of your aniline blue, evaporation will make it more concentrated. On the other hand a large volume of slides will weaken the working solutions. following is a link that lists some guidelines for storing stock solutions
Rena Fail www.urmc.rochester.edu/.../StainsManual/STABILITYOFSPECIALSTAININGSOLUTIONS.html?...- Cached ----- Original Message ---- From: "Sherwood, Margaret" <msherw...@partners.org> To: histonet@lists.utsouthwestern.edu Sent: Mon, August 9, 2010 10:32:48 AM Subject: [Histonet] Re: [Special Stains] Masson's Trichrome To all: We keep having the same discussion re: special stains. How long can you use them before discarding? I am specifically referring to the stains used in Masson's Trichrome. We are a research lab and, therefore, don't run the volume that most labs do. We have found, for instance, that we can use the Weigert's Hematoxylin (A&B) more than once, with filtering (even though it is stated it should be made fresh). We also use Bouin's Fix, Biebrich Scarlet and Aniline Blue more than once. Is there a "set rule" when we should discard these solutions (i.e. after 5X use or 1 month)? I would appreciate some feedback from the experts! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet