Mohamed,
Deposition of C4d is an inactive fragment of C4b (derived from complement component C4). It provides in-situ evidence of complement activation following alloantibody binding to graft vasculature. As such, the development of antibodies against C4d has dramatically improved the ability to diagnose antibody mediated rejection (AMR). We use the C4d (along with C3d) antibody from Cell Marque to investigate heart, lung and kidney biopsies for antibody mediated rejection in transplanted organs - in fact many consider C4d immunohistochemistry to be the gold standard for evaluation of AMR. C4d has also been postulated as a specific marker in the differential diagnosis of Follicular Lymphoma vs MALT lymphoma (Pathology - Research and Practice 2007; 203:163-167) Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org<mailto:ronald.hous...@nationwidechildrens.org> www.NationwideChildrens.org<http://www.NationwideChildrens.org> -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mohamed abd el razik Sent: Friday, August 13, 2010 1:46 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please enter Dear histonetters i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are ashamed to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector. so i put our proplem on your hands and my hope is to help us to find solution an example of what i'm taking about is what is C4d and for what it is used? thanx in advance to your help and for these amazing group mohamed Faculty of Vet. Med. Cairo Univ. - Egypt We use the same antibody from Cell Marque on the Dako Autostainers with great success. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald [ronald.hous...@nationwidechildrens.org] Sent: Friday, August 13, 2010 10:29 AM To: 'Martha Ward'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: C4d on paraffin sections We use the C4d from Cell Marque now with great results; it is an IVD. ER1 20 minutes on the BondMax and Bond III Ronnie Houston Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Friday, August 13, 2010 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d on paraffin sections I need some advice. We have been trying to do C4d on paraffin sections without much success. Does anyone have a good protocol we could try? We use the C4d from Quidel for our frozen sections and ideally I would like a procedure that we can use with our Bond Max, but I may be hoping for too much! Thanks in advance for your help. Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. 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