Hi, If you have a question the best thing to do is ask, so you are on the right track. I have three really good suggestions for you. First speak to your pathologists and try to learn as much as you possibly can from them about what they are looking for. This will familiarize you with what you need to know. Second, Read your antibody data sheets. Not just the parts of them that tell you how to do the procedure. Any (good) data sheet that should come with your antibodies will explain about the antibody, what it is, what it labels and which disease processes it is used for. Finally and very importantly use those autostainer companies until they get sick of you and then some more. (They like it when you do this ... really) They have some great literature that should help you. The new Cell Marque catalog is GREAT, it has images of each antibody on positive tissue, and great descriptions of each. DAKO has some of the best IHC guides out there. This is a link to a PDF of their Staining Methods Guide http://pri.dako.com/08002_ihc_staining_methods_5ed.pdf and they also have another really good book describing many clinically used antibodies with great images and descriptions. Unfortunately I can't recall the name of the book, if anyone can refresh my memory I'd appreciate it. There is a similar one for special stains. Now while I said three suggestions I'll toss in another anyway. The internet can be very helpful. Wikipedia can be useful for example in answer to your question about C4d, I found http://en.wikipedia.org/wiki/Complement_component_4
Have a great day, Amos Message: 1 Date: Fri, 13 Aug 2010 10:45:47 -0700 (PDT) From: mohamed abd el razik <k8...@yahoo.com> Subject: [Histonet] please enter To: Histonet@lists.utsouthwestern.edu Message-ID: <875537.15783...@web112609.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear histonetters i have a requist for all of you as i'm new in histochemistery and IHC as many other frinds i know in histonet . we found it difficult to know the name and use of many antibodies in many many topics so we can't understand the all masages and replaing on it. and lose the opportunity to learn from it!! and we are ashamed to talk about that with experts like you.but the group lose its functionality in teaching IHC for the new histologiest sector. so i put our proplem on your hands and my hope is to help us to find solution an example of what i'm taking about is what is C4d and for what it is used? thanx in advance to your help and for these amazing group mohamed Faculty of Vet. Med. Cairo Univ. - Egypt We use the same antibody from Cell Marque on the Dako Autostainers with great success. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet