Happy Friday all,
try 10% sodium hydroxide for 1 hour prior to processing. It is important to
let the slides drain really well before placing them in the oven. You want
to make sure there is no water between the section and the slide. We use
Plus slides with plain distilled water in our water baths. Hope this helps.
JTT
----- Original Message -----
From: "Andrew Burgeson" <nap...@siscom.net>
To: <histonet@lists.utsouthwestern.edu>
Sent: Friday, August 13, 2010 3:57 PM
Subject: [Histonet]
cf0145ebf1eb4c4e82768d82886a0c9b8fb...@pluto.ad.murdoch.edu.au
Joe "the toe" Nocito...are you out there?
Joe has good ideas about nails. Maybe he will send out his
procedure again.
I like using either potassium hydroxide 10-20% or Sodium
hydroxide 10-20% for softening nail fragments before
processing.
Also, keep in mind that soft tissues attached are equally as
important and sections of nail beds need to be of high
quality. A melanoma under a nail can be a bad situation.
Sometimes in addition to PAS or GMS stains for
onychomycosis, we have done melanin and iron stains for
areas of pigment or hemmorhagic depositions.
Joe...are you out there? lol
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