Hi everyone, I'm having a difficult time getting reliable structural staining for OCT embedded samples of mouse testes. Long story short, when I use 4% PFA (4C) for 2-4h, the connective tissue between the tubules of the testis is gone or noticeably shrunken. I need to visualize the Sertoli and Leydig cells within the testes since I'm looking for differences in antibody staining (Gproteins, hormone receptors) between different knockouts. I have yet to find a method for fixation that does not require antigen retrieval and also provides great structure.
Any advice is welcomed! Cheers, Rocky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
