Try fixing in cold acetone or "PenFix" (I think you can get it th rough Fisher or the likes). Acetone is what I usually use, although i= t will hurt some of the lipid-type structures. Pen Fix does contain a= small amount of formaldehyde, but it usually doesn't require any retrieval= ...good luck!!
Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg= 4 Suite 100 Austin, Texas 78744 <= em> (512)386-5= 107 -------- Original Message -------- Subject: [Histonet] Problems with fixation of mouse testes for IHC From: Rocky Parker <[1]rpar...@monell= .org> Date: Mon, August 30, 2010 12:09 pm To: [2]histo...@lists.uts= outhwestern.edu Hi everyone, I'm having a difficult time getting reliable structural staining for OCT embedded samples of mouse testes. Long story short, when I use 4% PFA (4C) for 2-4h, the connective tissue between the tubules of the testis is gone or noticeably shrunken. I need to visualize the Sertoli and Leydig cells within the testes since I'm looking for differences in antibody staining (Gproteins, hormone receptors) between different knockouts. I have yet to find a method for fixation that does not require antigen retrieval and also provides great structure. Any advice is welcomed! Cheers, Rocky _______________________________________________ Histonet mailing list [3]histo...@lists.utsouth= western.edu [4]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:rpar...@monell.org"; 2. 3D"mailto:histonet@lists.utsouthwestern.edu"; 3. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 4. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
