Thanks, Andrea! Is it imperative to rehydrate in the fixative or can I just go straight to rehydration in water? Is rehydrating in fixative because of the long dry-time because I don't see it in any protocols? Thanks again! --Teresa University of CA, Davis
On Tue, Aug 31, 2010 at 8:30 PM, <andreahoo...@rocketmail.com> wrote: > H+E staining should be unaffected by storage of frozen sections at RT. I > have done this for at least one week and its not a problem. It is IHC or > enzymatic stains which could pose a problem - depending on fixative etc. > > I do not suggest you rinse in PBS before airdrying, however. I take off > cryostat directly onto slide and air dry. Air dry cryosections in OCT and > leave at RT. When ready to stain, just rehydrate in your fixative for 5-10 > min then PBS or water and proceed. > > Sent from my Verizon Wireless BlackBerry > > -----Original Message----- > From: Teresa Iglesias <tligles...@ucdavis.edu> > Sender: histonet-boun...@lists.utsouthwestern.edu > Date: Tue, 31 Aug 2010 19:38:32 > To: <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] delaying Hematoxylin & Eosin staining? > > I'm a guest in a lab with very little freezer/fridge space so I'm wondering > if I can mount fixed-frozen testes tissue onto slides, and once dry, store > in a slide box at room temperature for a few weeks until all my slides are > ready for staining. > Tissues are fixed in 10% formalin then sunk in 20% sucrose. I'll embed in > OCT (optimal cutting temperature compound) before cryosectioning. I will > mount them after a rinse in PBS (phosphate buffered solution). > I worry about the tissues over drying since they are not embedded in > paraffin. Does anyone have any experience with this? > > Thanks! > -- > Teresa Iglesias > Graduate Group in Animal Behavior > University of California-Davis > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- ______________________________ Teresa Iglesias Graduate Group in Animal Behavior Department of Evolution and Ecology University of California-Davis One Shields Avenue 2320 Storer Hall Davis, CA 95616 Office: 530-754-7837 Fax: 530-752-1449 tligles...@ucdavis.edu ______________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet