Formalin fixation time does not impact the results of FISH as it does IHC. Jan M Omaha
-----Original Message----- From: Mike Pence [mailto:mpe...@grhs.net] Sent: Tuesday, October 12, 2010 11:00 AM To: Mahoney,Janice A; Joyce Cline; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR I don't think it matters if you do Her2 by FISH or IHC the time is still 48hr. I hope I am wrong, but I don't think I am. Mike -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A Sent: Tuesday, October 12, 2010 10:25 AM To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR We have decided to reflex to FISH those breasts that do not fall within the recommended formalin fixation time. We do work on Saturdays so it is only the rare 3 day weekends that this comes into play. Jan M Omaha -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Tuesday, October 12, 2010 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR Does anyone have any experience with storing formalin fixed breast tissue in 70% before processing? I am trying to comply with the new guidelines set forth by CAP and ASCO with regard to Her2 and ER/PR and since my lab does not operate on the weekend we have been well above the 48 hour recommended formalin fixation time. Does 70% affect antigenicity for either Her2 or ER/PR? Any information or suggestions will be greatly appreciated. Thanks :) Ronda Souders Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ronda.soud...@wchsys.org<mailto:ronda.soud...@wchsys.org> ________________________________ ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet