Nilesh: 1- if you are fixing with NBF do not think that because the biopsies are small require less time in the fixative. An "acceptable" fixing time will be 8 hours 2- the XpressX120 should give you good results IF the biopsies are well fixed. 3- Harris is a better hematoxylin because it is regressive and can be controlled better than Harris that is progressive and will need more staining time as it gets older/weaker René J.
--- On Tue, 10/12/10, Gupta, Nilesh <ngup...@hfhs.org> wrote: From: Gupta, Nilesh <ngup...@hfhs.org> Subject: [Histonet] Optimal processing for prostate needle biopsies To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Tuesday, October 12, 2010, 1:02 PM I have a few questions regrading processing of prostate needle biopsies. 1. What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2. Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3. What is the best H&E stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which H&E is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet