Phyllis, We hold all our needle cores overnight and pre-process for 15 min. The problems we are having is shrunken nuclei and nucleoli are not distinct even in carcinoma cases. Cracking artifacts of cores (longitudinal), darker and paler staining along the length of the cores. We tried Harris but staining with Mayer's brings out nucleoli better.
________________________________ From: Phyllis Thaxton [dch...@yahoo.com] Sent: Wednesday, October 13, 2010 10:37 AM To: Gupta, Nilesh; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Optimal processing for prostate needle biopsies How long are you pre-processing the prostate biopsies before processing on the XPress120? We use the XPress50 we fix the biopsies in Hollandes for 2 hours, then wash for 20 minutes, pre-processing solution for 10 minutes then process. We use Harris Hematoxylin in our H&E and morphology is good. Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ________________________________ From: "Gupta, Nilesh" <ngup...@hfhs.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Tue, October 12, 2010 12:02:23 PM Subject: [Histonet] Optimal processing for prostate needle biopsies I have a few questions regrading processing of prostate needle biopsies. 1. What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2. Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if any other labs have tried this processor for prostate needle cores processing and would like to know their experience as far as morphologic quality on slides 3. What is the best H&E stain to use for prostate needle biopsies. We are currently using Mayer's which is giving us better staining than Harris. Any recommendations on which H&E is best suited for prostate needle biopsies. Thanks N.Gupta Henry Ford Hospital ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com<http://www.henryford.com/> for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet