How do you define fixation time? A half pound hunk of fat with a tumor in the middle will remain unfixed until blocked. A small biopsy will start fixing almost immediately.
Bill At 1:51 PM -0400 10/13/10, Kuhnla, Melissa wrote: >Fixation prior to the processor will not exce4ed 12 hrs. That is why we >program the processor for 36...not to exceed 48. > > > >________________________________ > >From: Mike Pence [mailto:mpe...@grhs.net] >Sent: Wednesday, October 13, 2010 11:04 AM >To: Kuhnla, Melissa; Phyllis Thaxton; Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > >How much time is the tissue in formalin prior to going in the processor? >Your total time in formalin can not exceed 48 hr. And you will still >need to validate your process if you hold your tissue longer than >"normal processing" time in 70% (ie. more than 1 hr). > > -----Original Message----- > From: Kuhnla, Melissa [mailto:melissa.kuh...@chsli.org] > Sent: Wednesday, October 13, 2010 9:45 AM > To: Phyllis Thaxton; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > For the weekend, we have our processor set for 36 hours in >formalin and then a hold in 70%. This allows for complete fixation and >cuts down on prolonged time in 70% > > > > >________________________________ > > > From: Phyllis Thaxton [mailto:dch...@yahoo.com] > Sent: Wednesday, October 13, 2010 10:32 AM > To: Kuhnla, Melissa; Mahoney,Janice A; Mike Pence; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > > > > We run a weekend (Friday til Monday AM) breast run where the >tissues are in 10% NBF for 8 hours, then in 70% alcohol for 48 hours in >order to complete processing on Monday morning. So far no problems. > > > > Phyllis Thaxton HT(ASCP)QIHC > DCH Regional Medical Center > Tuscaloosa, AL > > > > > > >________________________________ > > > From: "Kuhnla, Melissa" <melissa.kuh...@chsli.org> > To: "Mahoney,Janice A" <janice.maho...@alegent.org>; Mike Pence ><mpe...@grhs.net>; Joyce Cline <joyce.cl...@wchsys.org>; >histonet@lists.utsouthwestern.edu > Sent: Tue, October 12, 2010 12:02:32 PM > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I disagree. Prolonged formalin fixation (over 48 hrs), >diminishes > signals > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 12:05 PM > To: 'Mike Pence'; Joyce Cline; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > Formalin fixation time does not impact the results of FISH as it >does > IHC. > Jan M > Omaha > > -----Original Message----- > From: Mike Pence [mailto:mpe...@grhs.net] > Sent: Tuesday, October 12, 2010 11:00 AM > To: Mahoney,Janice A; Joyce Cline; >histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: New Cap Guidelines for Her2 and >ER/PR > > I don't think it matters if you do Her2 by FISH or IHC the time >is still > 48hr. I hope I am wrong, but I don't think I am. > > Mike > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > Mahoney,Janice A > Sent: Tuesday, October 12, 2010 10:25 AM > To: 'Joyce Cline'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR > > > We have decided to reflex to FISH those breasts that do not fall >within > the recommended formalin fixation time. We do work on Saturdays >so it > is only the rare 3 day weekends that this comes into play. Jan M >Omaha > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of >Joyce > Cline > Sent: Tuesday, October 12, 2010 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] New Cap Guidelines for Her2 and ER/PR > > Does anyone have any experience with storing formalin fixed >breast > tissue in 70% before processing? I am trying to comply with the >new > guidelines set forth by CAP and ASCO with regard to Her2 and >ER/PR and > since my lab does not operate on the weekend we have been well >above the > 48 hour recommended formalin fixation time. Does 70% affect > antigenicity for either Her2 or ER/PR? Any information or >suggestions > will be greatly appreciated. Thanks :) > > > Ronda Souders > Hagerstown Medical Laboratory > 301-665-4980 > fax 301-665-4941 > ronda.soud...@wchsys.org<mailto:ronda.soud...@wchsys.org> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
