Thanks to everyone who responded. I removed the coverslip, and ran it back to water and re applied the secondary, dab and counterstain. It worked like a charm.
Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. >>> "Kimberly Tuttle" <ktut...@umm.edu> 10/13/2010 12:07 pm >>> Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
