To clarify even further: we cut 2 patient sections, put one on a slide with a section of positive control already on it. This slide gets stained with the antibody The other section goes on another slide and is run as a corresponding negative control using the same antibody protocol but substituting a negative control serum for the antibody, thus this is a "negative reagent control" slide. Elements within the patient slide that received antibody and are expected to be negative, serve as a "negative tissue control". Again, we run 1 negative control slide for EVERY 1 patient block in a run but only 1 negative control per any number of antibodies run on that same block, using the harshest protocol. Only autopsy cases differ in that we run 1 negative control per TISSUE TYPE.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [email protected] [mailto:[email protected]] Sent: Friday, October 15, 2010 10:48 AM To: Rene J Buesa Cc: Histo Net list server; Sebree Linda A Subject: RE: [Histonet] negative controls So for every HP you do, you process a control cassette with the patient tissue cassette? That seems like alot? How do you get that many control tissues on a daily basis? What do you do with the remaining tissue in the control block? If you throw them away everyday, I would be interested in some of them. How do you know what IHC stains the pathologist is going to order to know what control tissue to fix and process at the exact same time? We have always just had a bunch of blocks that you cut a control from? I understand that there is variability with processing, age, etc. not trying to be dense just still don't understand... Most places I have ever worked have control blocks that they cut a fresh control from everyday, then stain with the patient tissue. If there are 3 HP cases, from what I am understanding, you guys are saying you need 3 controls for slides that are on the same machine, with the same reagents, same antibody, and same times. Why couldn't you just have one for all 3 cases? Then the next day have a fresh ONE for that day, date them, and file them. So if you needed to see the HP control for October 15th, you could go pull the control for that day... Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100 Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: Rene J Buesa <[email protected]> Date: Fri, October 15, 2010 8:33 am To: Sebree Linda A <[email protected]>, [email protected] Cc: Histo Net list server <[email protected]> Because each tissue block has its own characteristics regarding fixation and processing some of which can influence the reactivity. If you have a bank of negative controls, how can you be sure that any of those blocks have received exactly the same treatment and reacted in the same way to the test block? The same goes for any bank of positives, so that is why you should have a positive control section in the same slide as the test section. René J. --- On Fri, 10/15/10, [email protected] <[email protected]> wrote: From: [email protected] <[email protected]> Subject: RE: [Histonet] negative controls To: "Sebree Linda A" <[email protected]> Cc: "Histo Net list server" <[email protected]> Date: Friday, October 15, 2010, 11:17 AM Why do you need a negative control for each block if you are runn= ing the same antibody on each patient block? Is it just for case by c ase reference so the negative is filed with the patient slide? Why co= uldn't you have a control slide bank that was dated so all the slides you d= id on that day, on that run, could be referenced back to that control? = ; Just curious? Sarah Goebel, B.A., HT (ASCP) Histotechnician<= br> XBiotech USA Inc. 8201 East Riverside Dr. Bld= g 4 Suite 100 Austin, Texas 78744 = (512)386-= 5107 -------- Original Message -------- Subject: RE: [Histonet] negative controls From: "Sebree Linda A" <[1]lseb...@= uwhealth.org> Date: Fri, October 15, 2010 8:08 am To: "Victoria Baker" <[2]bakevict= [email protected]>, "Histo Net list server" <[3][email protected]= outhwestern.edu> We run negative controls on every block of a case within the same run. On autopsy cases, we only run 1 negative per tissue type, within the same run...this is the only exception to the rule of 1 negative per block. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [4]histonet= [email protected] [[5]mailto:histon= [email protected]] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 9:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list [6][email protected]= western.edu [7]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [8][email protected]= western.edu [9]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:[email protected]"; 2. 3D"mailto:[email protected]"; 3. 3D"mailto:[email protected]"; 4. 3D"mailto:[email protected]"; 5. 3D"mailto:[email protected]"; 6. 3D"mailto:[email protected]"; 7. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; 8. 3D"mailto:[email protected]"; 9. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
