I run both positive and negative controls. Wherever possible, I put the positive control on the same slide as the test patient (this makes it easier for the pathologist and it also saves us the cost of on slide being tested). I always run a negative patient control on the exact same run. If I have more slides than will fit on one run, I will hold the specific case back, as I won't separate them. Matters not if there is more than one machine, the case will stay together.
As far as negatives, I will run an extra negative if my run has both poly and mono antibodies. So, there are many times, I have two negatives running per case. I run only one detection system, so I don't have that variable to deal with. Michelle -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Friday, October 15, 2010 12:50 PM To: Victoria Baker; Histo Net list server Subject: RE: [Histonet] negative controls Hi Vikki, I have 1 Ventana XT and 3 Ultras. I have certain antibodies designated to each ultra. This means not all slides from any case are guaranteed to be on the same machine. They obviously all have their own detection kit. My theory is that our detection kits are QCd prior to initial load onto an instrument. The patient block was treated the same prior to cutting. The slides receive solutions that are QCd...I think we are covered. What are your thoughts? Melissa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, October 15, 2010 10:26 AM To: Histo Net list server Subject: [Histonet] negative controls Hi I have a hypothetical question to those who run IHC on Ventana instruments. Are you running your negatives with your patient/test cases or on a separate run? Also, if you are doing this and have to use a different detection kit how do you work the QA/QC portion of this for CAP requirements. Thanks Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.5.448 / Virus Database: 271.1.1/3183 - Release Date: 10/13/10 18:34:00 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet