Hi all, Does anyone have a protocol for whole rat brain immersion fixation using 4% Paraformaldehyde for IHC where no transcardial perfusion has been performed? I am specifically interested in knowing how long does the brain need to be immersed for a good complete fixation before transferring to sucrose. Also, I would like a protocol for rat brain tissue transcardial perfusion fixation down to freezing to sectioning if possible to compare with my current protocol. Thanks!
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