Dear Histology Professionals, I am using the Azure B as a counter-stain for melanin preparing solution by the protocol below. My questions are: 1. Is this solution should be fresh prepared every day? 2. How long I can store and use this solution? 3. Where this solution should be stored in the room or in the refrigerator?
My protocol is: Counterstain Method The use of Azure B as a counter-stain will stain melanin a blue green color. Azure B working reagent: 4mL of a 25mg/mL aqueous solution of Azure B 3.4mL of 0.1M acetic acid 600uL of 0.1M sodium acetate 27mL of DI water 5mL acetone Incubate for up to 30 minutes, followed by a 20-second hematoxylin counter-stain. The use of either counterstain should give you good cytological detail, with blue-green melanin and brown DAB specific signal. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet