Naira, we use a standard working Giemsa solution that is good for >1 month, stored at 4C. We automate the procedure on our Bonds: 3min Giemsa and 1 min Hematoxylin.
Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org<mailto:ronald.hous...@nationwidechildrens.org> www.NationwideChildrens.org<http://www.NationwideChildrens.org> -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Friday, February 11, 2011 11:22 AM To: histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] Azure B as a counter-stain for melanin Importance: High Dear Histology Professionals, I am using the Azure B as a counter-stain for melanin preparing solution by the protocol below. My questions are: 1. Is this solution should be fresh prepared every day? 2. How long I can store and use this solution? 3. Where this solution should be stored in the room or in the refrigerator? My protocol is: Counterstain Method The use of Azure B as a counter-stain will stain melanin a blue green color. Azure B working reagent: 4mL of a 25mg/mL aqueous solution of Azure B 3.4mL of 0.1M acetic acid 600uL of 0.1M sodium acetate 27mL of DI water 5mL acetone Incubate for up to 30 minutes, followed by a 20-second hematoxylin counter-stain. The use of either counterstain should give you good cytological detail, with blue-green melanin and brown DAB specific signal. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet